Anti-aging effects of fetal dermal mesenchymal stem cells in a D-galactose-induced aging model of adult dermal fibroblasts

The main characteristic of skin aging is the change in the composition of the dermis, mainly resulting from fibroblast senescence. Mesenchymal stem cells derived from fetal dermis are defined as fetal dermal mesenchymal stem cells; they reportedly exert wound healing effects on the skin and regulate...

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Bibliographic Details
Published in:In vitro cellular & developmental biology. Animal 2021-09, Vol.57 (8), p.795-807
Main Authors: Pan, Shengsheng, Gong, Siyu, Zhang, Jingjuan, Jia, Shanshan, Wang, Maoying, Pan, Yi, Wang, Xiao, Jiang, Duyin
Format: Article
Language:English
Subjects:
Adult
Aging
Aging (artificial)
Aging - drug effects
Aging - physiology
Animal Genetics and Genomics
Animal models
Animals
Apoptosis
Biomedical and Life Sciences
Blotting, Western
Cell Biology
Cell Culture
Cellular Senescence - drug effects
Culture Media
D-Galactose
Dermis
Dermis - cytology
Dermis - embryology
Developmental Biology
Fetuses
Fibroblasts
Fibroblasts - drug effects
Fibroblasts - physiology
Galactose
Galactose - pharmacology
Galactosidase
Gene expression
Humans
Life Sciences
Male
Mesenchymal stem cells
Mesenchymal Stem Cells - physiology
Paracrine signalling
Reactive oxygen species
Reactive Oxygen Species - metabolism
Reverse Transcriptase Polymerase Chain Reaction
Senescence
Skin
Stem cell transplantation
Stem Cells
Wound healing
β-Galactosidase
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Summary:The main characteristic of skin aging is the change in the composition of the dermis, mainly resulting from fibroblast senescence. Mesenchymal stem cells derived from fetal dermis are defined as fetal dermal mesenchymal stem cells; they reportedly exert wound healing effects on the skin and regulate keloid fibroblast proliferation. D-Galactose is widely used in animal aging models. In this study, we confirmed that D-galactose inhibits adult dermal fibroblast proliferation, and the inhibitory effect gradually increased with increasing concentration. Finally, we chose a concentration of 40 g/L D-galactose to induce adult dermal fibroblast senescence. D-Galactose increased the intensity of senescence-associated β-galactosidase staining and the levels of reactive oxygen species in adult dermal fibroblasts. Furthermore, D-galactose increased the mRNA expression of p16 , p21 , and p53 . The fetal dermal mesenchymal stem cell–conditioned medium improved the above-mentioned effects. Overall, fetal dermal mesenchymal stem cells exerted anti-aging effects against adult dermal fibroblasts induced by D-galactose via paracrine functions.
ISSN:1071-2690
1543-706X
DOI:10.1007/s11626-021-00624-z