Genomic characterization of a rare Carica papaya X chromosome mutant reveals a candidate monodehydroascorbate reductase 4 gene involved in all-hermaphrodite phenomenon

Sex form is one of the most important characteristics in papaya cultivation in which hermaphrodite is the preferable form. Self-pollination of H*-TSS No.7, an inbred line derived from a rare X chromosome mutant SR*, produced all-hermaphrodite progeny. The recessive lethal allele controlling the all-...

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Published in:Molecular genetics and genomics : MGG 2021-11, Vol.296 (6), p.1323-1335
Main Authors: Chen, Jen-Ren, Ueno, Hiroki, Matsumura, Hideo, Urasaki, Naoya, Lee, Chen-Yu, Chen, Fure-Chyi, Chin, Shih-Wen, Liu, Chun-Chi, Chiu, Chan-Tai, Tarora, Kazuhiko, Li, Jing-Yi, Lee, Chieh Ying, Ku, Hsin-Mei
Format: Article
Language:English
Subjects:
Alleles
Animal Genetics and Genomics
Biochemistry
Biomedical and Life Sciences
Carica papaya
Gene deletion
Hermaphrodites
Human Genetics
Hydrogen peroxide
Inbreeding
Life Sciences
Microbial Genetics and Genomics
Mutants
Next-generation sequencing
Original Article
Plant Genetics and Genomics
Pollination
Seed germination
Seeds
X chromosomes
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Summary:Sex form is one of the most important characteristics in papaya cultivation in which hermaphrodite is the preferable form. Self-pollination of H*-TSS No.7, an inbred line derived from a rare X chromosome mutant SR*, produced all-hermaphrodite progeny. The recessive lethal allele controlling the all-hermaphrodite phenomenon was proposed to be the recessive Germination suppressor ( gs ) locus. This study employed next-generation sequencing technology and genome comparison to identify the candidate Gs gene. One specific gene, monodehydroascorbate reductase 4 ( MDAR4 ) harboring a unique polymorphic 3 bp deletion in H*-TSS No.7 was identified. The function of MDAR4 is known to be involved in the hydrogen peroxide (H 2 O 2 ) scavenging pathway and is associated with seed germination. Furthermore, MDAR4 showed higher expression in the imbibed seeds than that in the dry seeds indicating its potential role in the seed germination. Perhaps this is the very first report providing the evidences that MDAR4 is the candidate of Gs locus in H*-TSS No.7. In addition, Gs allele-specific markers were developed which would be facilitated for breeding all-hermaphrodite lines.
ISSN:1617-4615
1617-4623
DOI:10.1007/s00438-021-01822-w