Heat shock factor 1 in brain tumors: a link with transient receptor potential channels TRPV1 and TRPA1

Novel data report a “cross-talk” between Heat-Shock Factor 1 (HSF1) and the transient receptor potential vanilloid 1 cation channel (TRPV1) located in the cell membrane, introducing these channels as possible drug targets for the regulation of HSF1 activation. This study aims to investigate the co-e...

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Bibliographic Details
Published in:	Journal of molecular histology 2021-12, Vol.52 (6), p.1233-1244
Main Authors:	Moutafidi, Athanasia, Gatzounis, George, Zolota, Vassiliki, Assimakopoulou, Martha
Format:	Article
Language:	eng
Subjects:	Adult Aged Aged, 80 and over Ankyrin Biomarkers Biomedical and Life Sciences Biomedicine Brain cancer Brain Neoplasms - diagnosis Brain Neoplasms - etiology Brain Neoplasms - metabolism Brain tumors Capsaicin receptors Case-Control Studies Cell Biology Cell membranes Correlation analysis Developmental Biology Disease Susceptibility Female Gene Expression Glioma Heat shock factors Heat Shock Transcription Factors - genetics Heat Shock Transcription Factors - metabolism HSF1 protein Humans Immunohistochemistry Life Sciences Male Meningioma Middle Aged mRNA Neoplasm Grading Neoplasm Staging Organ Specificity Original Paper Paraffin Prognosis Protein Binding Signal Transduction Therapeutic targets Transient receptor potential proteins TRPA1 Cation Channel - metabolism TRPV Cation Channels - metabolism
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Summary:	Novel data report a “cross-talk” between Heat-Shock Factor 1 (HSF1) and the transient receptor potential vanilloid 1 cation channel (TRPV1) located in the cell membrane, introducing these channels as possible drug targets for the regulation of HSF1 activation. This study aims to investigate the co-expression of TRPV1 and HSF1 in human brain tumors. Additionally, the expression of the transient receptor potential ankyrin 1 channel (TRPA1), which is co-operated with TRPV1 in a plethora of cells, was studied. Immunohistochemical staining for HSF1, TRPV1 and TRPA1 expression was quantitatively analyzed in paraffin-embedded semi-serial tissue sections from 74 gliomas and 71 meningiomas. mRNA levels of HSF1, TRPV1 and TRPA1 were evaluated using real-time PCR. Although HSF1 was significantly increased compared with TRPV1/TRPA1 (p ≤ 0.001) in both gliomas and meningiomas, high co-expression levels for HSF1, TRPV1 and TRPA1 were found in 62.50% of diffuse fibrillary astrocytomas (WHO, grade II), 37.50% of anaplastic astrocytomas (WHO, grade III), 16.32% of glioblastomas multiforme (WHO, grade IV), and 42.25% of meningiomas (WHO, grade I and II). Correlation analysis revealed a relationship of HSF1 with TRPV1/TRPA1 in diffuse fibrillary astrocytomas (WHO, grade II) and benign meningiomas (WHO, grade I) contrary to glioblastomas multiforme (WHO, grade IV) and high grade meningiomas (WHO, grade II). Importantly, TRPA1 and TRPV1 expression levels were significantly increased in meningiomas compared with astrocytic tumors (p
ISSN:	1567-2379 1567-2387