Sulphur-doped graphene quantum dot based fluorescent turn-on aptasensor for selective and ultrasensitive detection of omethoate

Development of selective, ultra-sensitive, rapid and facile methods for the detection of chemical residues of toxic pesticides and hazardous chemicals are quite important in food safety, environmental monitoring and safeguarding public health. Herein, we presented a fluorescent turn-on aptasensor ba...

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Bibliographic Details
Published in:Analytica chimica acta 2021-10, Vol.1181, p.338893-338893, Article 338893
Main Authors: Nair, Raji V., Chandran, Parvathy R., Mohamed, A. Peer, Pillai, Saju
Format: Article
Language:English
Subjects:
Aggregation-disaggregation
Aptasensor
Fluorescence ‘turn-on’
Omethoate
Specific recognition
Sulphur-doped graphene quantum dots
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Summary:Development of selective, ultra-sensitive, rapid and facile methods for the detection of chemical residues of toxic pesticides and hazardous chemicals are quite important in food safety, environmental monitoring and safeguarding public health. Herein, we presented a fluorescent turn-on aptasensor based on sulphur-doped graphene quantum dot (S-GQD) utilizing specific recognition and binding property of aptamer for the ultra-sensitive and selective detection of omethoate (OM) which is a systemic organophosphorus pesticide. The detection method is based on tuning aggregation-disaggregation mechanism of S-GQD by way of conformational alteration of the recognition probe. Fluorescence ‘turn-on’ process includes aggregation-induced quenching of S-GQD with aptamer via S-GQD-aptamer complex formation and its subsequent fluorescence recovery with the addition of OM by structural switching of S-GQD-aptamer complex to aptamer-omethoate complex. The reported ‘switch-on’ aptasensor has exhibited a low limit of detection of 0.001 ppm with high selectivity for OM over other pesticides. [Display omitted] •Sulphur-doped graphene quantum dot-based fluorescence turn-on aptasensor was demonstrated for the detection of omethoate.•Detection method was based on conformational alteration of aptamer that mediated aggregation and disaggregation of S-GQD.•Sensing strategy was not demanding the modification of either the signal transmitting nanoparticle or the reporter probe.•The sensor exhibited very low limit of detection (1 ppb) and high selectivity towards omethoate.
ISSN:0003-2670
1873-4324
DOI:10.1016/j.aca.2021.338893