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Inhibitors of protein kinases A and C impair the motility of oncospheres of the model tapeworm Hymenolepis microstoma
[Display omitted] Highlights •Optimization of hatching and activation of Hymenolepis microstoma oncospheres•Development of a motility assay for Hymenolepis microstoma oncospheres•Protein kinase C pathway inhibition impairs oncospheral motility•The protein kinase A inhibitor H-89 potently blocks onco...
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Published in: | Molecular and biochemical parasitology 2021-11, Vol.246, p.111423-111423, Article 111423 |
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Main Authors: | , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites |
Online Access: | Get full text |
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Highlights •Optimization of hatching and activation of Hymenolepis microstoma oncospheres•Development of a motility assay for Hymenolepis microstoma oncospheres•Protein kinase C pathway inhibition impairs oncospheral motility•The protein kinase A inhibitor H-89 potently blocks oncosphere motility
The oncosphere larvae of tapeworms cyclically extend and retract their hooks during the penetration of the intestine of their intermediate hosts. The mechanisms regulating these movements are essentially unknown, in part due to the biohazardous nature of oncospheres from human pathogens. In this work, we standardized a method for the analysis of motility of hatched oncospheres (hexacanths) of the model tapeworm Hymenolepis microstoma. We used this assay to explore the relevance of protein kinases C (PKC) and A (PKA) in these processes. Pharmacological inhibition of the PKC pathway resulted in impaired larval motility. On the other hand, the PKA inhibitor H-89 potently blocked larval motility, as well as the motility of other life stages, although other inhibitors of the PKA pathway were not effective. This work represents the first study of the mechanisms that regulate the motility of oncospheres, and provides a path for further exploration. |
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ISSN: | 0166-6851 1872-9428 |
DOI: | 10.1016/j.molbiopara.2021.111423 |