Nanosensing colon cancer biomarker on zeolite‐modified gap‐fingered dielectrodes

Nanomaterial on the sensing area elevates the biomolecular immobilization by its right orientation with a proper alignment, and zeolite is one of the suitable materials. In this research, the zeolite nanoparticles were synthesized using rice hush ash as the basic source and the prepared zeolite by t...

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Bibliographic Details
Published in:Biotechnology and applied biochemistry 2022-10, Vol.69 (5), p.1885-1892
Main Authors: Gao, Ming, Sun, Yuansong, Wang, Qi, Ma, Shuaiting, Guo, Xinwei, Zhou, Lingling, Chen, Yeng, Marimuthu, Kasi, Gopinath, Subash C.B.
Format: Article
Language:English
Subjects:
Aldehydes
Aluminum oxide
Antibodies
Biomarkers
Cancer
cancer stages
Colon
Colon cancer
colon cancer secreted protein‐2
Colorectal cancer
dielectrode
Electron microscopy
Field emission microscopy
Immobilization
Microscopy
Nanomaterials
Nanoparticles
Proteins
Scanning electron microscopy
Silica
silica‐aluminate
Sodium silicates
Transmission electron microscopy
Zeolites
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Summary:Nanomaterial on the sensing area elevates the biomolecular immobilization by its right orientation with a proper alignment, and zeolite is one of the suitable materials. In this research, the zeolite nanoparticles were synthesized using rice hush ash as the basic source and the prepared zeolite by the addition of sodium silicate was utilized to attach antibody as a probe on a gap‐fingered dielectrode surface to identify the colon cancer biomarker, “colon cancer‐secreted protein‐2” (CCSP‐2). Field Emission Scanning Electron Microscopy and Field Emission Transmission Electron Microscopy images confirmed the size of the nanoparticle to be ∼15 nm and the occurrence of silica and alumina. Zeolite was modified on the electrode surface through the amine linker, and then anti‐CCSP‐2 was attached by an aldehyde linker. On this surface, CCSP‐2 was detected and attained the detection limit to be 3 nM on the linear regression curve with 3–5 nM of CCSP‐2. Estimated by the determination coefficient of y = 2.3952x – 4.4869 and R2 = 9041 with 3δ (n = 3). In addition, control proteins did not produce the notable current response representing the specific sensing of CCSP‐2. This research is suitable to identify CCSP‐2 at a lower level in the bloodstream under the physiological condition of a colon cancer patient. Biosensing Colon Cancer Secreted Protein‐2.
ISSN:0885-4513
1470-8744
DOI:10.1002/bab.2254