The influence of probiotics of different microbiological composition on histology of the gastrointestinal tract of juvenile Oncorhynchus mykiss

ABSTRACT This article presents results of the influence of three probiotic feed additive of various microbiological composition: Bacillus subtilis (VKPM B‐2335); B. subtilis (OZ‐2 VKPM‐11966) + Bacillus amyloliquefaciens (OZ‐3 VKPM‐11967); Lactobacillus acidophilus (VKPM B‐3235) on the growth and hi...

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Published in:Microscopy research and technique 2022-02, Vol.85 (2), p.538-547
Main Authors: Nikiforov‐Nikishin, Alexei, Nikiforov‐Nikishin, Dimitri, Kochetkov, Nikita, Smorodinskaya, Svetlana, Klimov, Victor
Format: Article
Language:English
Subjects:
Additives
Animal Feed - analysis
Animals
Appendages
Aquaculture
Bacillus amyloliquefaciens
Bacillus subtilis
Composition
Diet
Epithelium
Feed additives
Food additives
Gastrointestinal system
Gastrointestinal Tract
Goblet cells
Histology
Immune response
intestinal histology
Intestine
Lactobacilli
Lactobacillus acidophilus
Lamina propria
Lymphocytes
Oncorhynchus mykiss
Organs
Parameters
Probiotics
Probiotics - pharmacology
Salmon
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Summary:ABSTRACT This article presents results of the influence of three probiotic feed additive of various microbiological composition: Bacillus subtilis (VKPM B‐2335); B. subtilis (OZ‐2 VKPM‐11966) + Bacillus amyloliquefaciens (OZ‐3 VKPM‐11967); Lactobacillus acidophilus (VKPM B‐3235) on the growth and histology of the organs of the gastrointestinal tract of juvenile Oncorhynchus mykiss by morphometric parameters. These probiotic bacteria are the most commonly used in aquaculture. The effect of the probiotic feed additive led to the increase in fish growth and influenced different sections of the gastrointestinal tract. The biggest change was found in the mid intestine and the reliable difference compared with the control diet was obtained at the following parameters: lamina propria width, intraepithelial lymphocytes number of prismatic epithelium and goblet cells area. The changes in the pyloric appendages were less obvious but reported as playing an important functional role in digestion. The liver preserved normal functional structure in all series of the experiment except for the group with L. acidophilus, where hepatocyte small‐drop vacuolization was observed. That might be connected with the change of the digest activity resulting from a decrease in secretory activity of the intestinal exocrinocytes. The use of all probiotic feed additives led to a similar change in morphometric parameters in all groups, which suggests a decrease in the immune response. Probiotic cause histomorphological changes in the intestine, which can be assessed by morphometric methods The effect of the studied probiotic caused significant changes in the number of goblet cells and intraepithelial lymphocytes The positive effect of complex probiotics has significantly improved the architectonics of the liver
ISSN:1059-910X
1097-0029
DOI:10.1002/jemt.23927