Interstitial collagenase MMP-1 and EMMPRIN in cell lines and in clinical specimens of cervical squamous cell carcinoma

Background The aim of this study was to elucidate the features of the expression of matrix metalloproteinases inducer—EMMPRIN (EMN) and matrix metalloproteinase 1 (MMP-1) in cell lines and in clinical samples of cervical squamous cell carcinoma (SCC). Material and methods The study was carried out u...

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Bibliographic Details
Published in:Molecular biology reports 2021-10, Vol.48 (10), p.6879-6886
Main Authors: Solovyeva, Nina I., Timoshenko, Olga S., Kugaevskaya, Elena V., Gureeva, Tatyana A.
Format: Article
Language:English
Subjects:
Animal Anatomy
Animal Biochemistry
Basigin - genetics
Basigin - metabolism
Biomedical and Life Sciences
Carcinoma, Squamous Cell - genetics
Carcinoma, Squamous Cell - metabolism
Carcinoma, Squamous Cell - pathology
Cell Line, Tumor
Cervical cancer
Cervix
Collagen
Collagenase
Densitometry
Female
Gene expression
Gene Expression Regulation, Neoplastic
Histology
Humans
Interstitial collagenase
Invasiveness
Life Sciences
Matrix metalloproteinase
Matrix Metalloproteinase 1 - genetics
Matrix Metalloproteinase 1 - metabolism
Metalloproteinase
Metastases
Morphology
Original Article
Polymerase chain reaction
RNA, Messenger - genetics
RNA, Messenger - metabolism
Squamous cell carcinoma
Uterine Cervical Neoplasms - genetics
Uterine Cervical Neoplasms - metabolism
Uterine Cervical Neoplasms - pathology
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Summary:Background The aim of this study was to elucidate the features of the expression of matrix metalloproteinases inducer—EMMPRIN (EMN) and matrix metalloproteinase 1 (MMP-1) in cell lines and in clinical samples of cervical squamous cell carcinoma (SCC). Material and methods The study was carried out using RT-PCR, densitometry and immunohistochemical studies (IHC) on commercial cell lines Siha, Caski, transformed with HPV16; HeLa, and C33A transformed with HPV18, line C33A without HPV, and in clinical samples of SCC and morphologically normal tissue adjacent to the tumor. Results The data obtained indicate that the expression of mRNA EMN and MMP-1 occurs in all cell lines at different levels. HPV type and number of genes copies had no effect on expression degree both EMN and MMP-1. Gene expression of EMN and MMP-1 has been investigated in tumor and normal tissues. MMP-1 expression in tumor tissue in SCC, as a rule, has been significantly increased (2–6 times) compared to normal tissue. It was found in 90% of tumor samples. It is known, that MMP-1 promotes the development of invasive and metastatic processes. EMN expression was lower in the tumor tissue than in normal tissue in most cases. An increase in EMN expression was noted only in some cases of SCC. Conclusion The data obtained indicate that MMP-1 can serve as a marker of the invasive potential of SCC. EMN, apparently, is not a major factor responsible for MMP-1 expression in SCC. Data are important for understanding the process of tumor development and may have prognostic value for the patient.
ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-021-06689-z