Contrast-enhanced ultrasound for evaluation of tumor perfusion and outcome following treatment in a murine melanoma model

•CEUS revealed a decrease in tumor perfusion aftersilencing MCAM and irradiation.•CEUS results correlated with decreased vascular density.•Decreased perfusion correlated with higher antitumor effectiveness. Due to a lack of data on predictors of electroporation-based treatment outcomes, we investiga...

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Bibliographic Details
Published in:Bioelectrochemistry (Amsterdam, Netherlands) Netherlands), 2021-12, Vol.142, p.107932-107932, Article 107932
Main Authors: Brloznik, Maja, Boc, Nina, Cemazar, Maja, Bosnjak, Masa, Savarin, Monika, Kejzar, Natasa, Sersa, Gregor, Pavlin, Darja, Kranjc Brezar, Simona
Format: Article
Language:English
Subjects:
Animals
Anticancer properties
Antitumor activity
Antivascular effects
Blood vessels
Cell adhesion
Cell adhesion molecules
Cell Line, Tumor
Combined treatment
Contrast-enhanced harmonic ultrasound
Density
Electroporation
Electroporation - methods
Female
Gene electrotransfer
Gene transfer
Irradiation
Melanoma
Melanoma cell adhesion molecule
Melanoma, Experimental - radiotherapy
Melanoma, Experimental - ultrastructure
Mice
Mice, Inbred C57BL
Murine melanoma B16F10
Perfusion
Radiation therapy
Silence
Tumors
Ultrasonic imaging
Ultrasonography - methods
Ultrasound
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Summary:•CEUS revealed a decrease in tumor perfusion aftersilencing MCAM and irradiation.•CEUS results correlated with decreased vascular density.•Decreased perfusion correlated with higher antitumor effectiveness. Due to a lack of data on predictors of electroporation-based treatment outcomes, we investigated the potential predictive role of contrast-enhanced harmonic ultrasound (CEUS) in mice B16F10 melanoma treated by gene electrotransfer (GET) to silence melanoma cell adhesion molecule (MCAM) and radiotherapy, which has not been evaluated yet. CEUS evaluation was verified by tumor histological analysis. Mice bearing subcutaneous tumors were treated with GET to silence MCAM, irradiation or the combination of GET to silence MCAM and irradiation (combined treatment). CEUS of the tumors used to evaluate tumor perfusion was performed before and up to 10 days after the beginning of the experiment, and the CEUS results were compared with tumor growth and the number of blood vessels analyzed in the histological tumor sections. CEUS revealed a decrease in tumor perfusion in the combined therapy groups compared with the control groups and correlated with tumor histological analyses, which showed a decreased vascular density. In this study a trend of inverse correlation was observed between tumor perfusion and treatment efficacy. The greater the perfusion of the tumor, the shorter the expected doubling time. Furthermore, decreased perfusion showed a trend to correlate with higher antitumor efficacy. Thus, CEUS could be used to predict tumoral vascular density and treatment effectiveness.
ISSN:1567-5394
1878-562X
DOI:10.1016/j.bioelechem.2021.107932