Overexpression of 4-hydroxyphenylpyruvate dioxygenase (IbHPPD) increases abiotic stress tolerance in transgenic sweetpotato plants

Tocopherols are lipid-soluble compounds regarded as vitamin E compounds and they function as antioxidants in scavenging lipid peroxyl radicals and quenching reactive oxygen species (ROS). In our previous studies, we isolated five tocopherol biosynthesis genes from sweetpotato (Ipomoea batatas [L.] L...

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Published in:Plant physiology and biochemistry 2021-10, Vol.167, p.420-429
Main Authors: Kim, So-Eun, Bian, Xiaofeng, Lee, Chan-Ju, Park, Sul-U, Lim, Ye-Hoon, Kim, Beg Hab, Park, Woo Sung, Ahn, Mi-Jeong, Ji, Chang Yoon, Yu, Yang, Xie, Yizhi, Kwak, Sang-Soo, Kim, Ho Soo
Format: Article
Language:English
Subjects:
Abiotic stress
Herbicide stress
IbHPPD
Sweetpotato
Tocopherol
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Summary:Tocopherols are lipid-soluble compounds regarded as vitamin E compounds and they function as antioxidants in scavenging lipid peroxyl radicals and quenching reactive oxygen species (ROS). In our previous studies, we isolated five tocopherol biosynthesis genes from sweetpotato (Ipomoea batatas [L.] Lam) plants including 4-hydroxyphenylpyruvate dioxygenase (IbHPPD). HPPD is the first regulatory enzyme in vitamin E biosynthesis and serves to catalyze in the first steps α-tocopherol and plastoquinone biosynthesis by converting 4-hydroxyphenylpyruvate (HPP) to homogentisic acid (HGA). In this study, we generated transgenic sweetpotato plants overexpressing IbHPPD under the control of cauliflower mosaic virus (CaMV) 35S promoter (referred to as HP plants) via Agrobacterium-mediated transformation to understand the function of IbHPPD in sweetpotato. Three transgenic lines (HP3, HP14 and HP15) with high transcript levels of IbHPPD were selected for further characterization. Compared with non-transgenic (NT) plants, HP plants exhibited enhanced tolerance to multiple environmental stresses, including salt, drought, and oxidative stresses. In addition, HP plants showed increased tolerance to the herbicide sulcotrione, which is involved in the inhibition of the HPPD. Interestingly, after stress treatments, HP plants also showed higher abscisic acid (ABA) contents than NT plants. Under dehydrated condition, HP plants displayed an elevated α-tocopherol content to 19–27% in leaves compared with NT plants. These results indicate that increased abiotic stress tolerance in HP plants is related to inducing enhancement of α-tocopherol and ABA contents. •Transgenic sweetpotato plants overexpressing IbHPPD (HP plants) were generated and characterized.•HP plants showed enhanced tolerance to multiple abiotic stresses, including salt, drought, oxidative and herbicide stresses.•HP plants exhibited elevated abscisic acid (ABA) contents and α-tocopherol content in leaves compared with NT plants.
ISSN:0981-9428
1873-2690
DOI:10.1016/j.plaphy.2021.08.025