Saccharomyces Cerevisiae as an Untapped Source of Fungal Chitosan for Antimicrobial Action

Despite being widely available, Saccharomyces cerevisiae has not been widely explored for direct extraction of chitosan biopolymer for antimicrobial applications. In our study, S. cerevisiae from Baker’s yeast and Aspergillus niger from moldy onion extracts are studied as alternative sources of chit...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 2021-11, Vol.193 (11), p.3765-3786
Main Authors: Afroz, Md. Masirul, Kashem, Md. Nayeem Hasan, Piash, K. M. Prottoy Shariar, Islam, Nafisa
Format: Article
Language:English
Subjects:
Acetic acid
Agricultural wastes
Anti-Infective Agents - chemistry
Anti-Infective Agents - pharmacology
Antiinfectives and antibacterials
Antimicrobial agents
Aspergillus niger
Aspergillus niger - chemistry
Biochemistry
Biomass
Biopolymers
Biotechnology
Chemistry
Chemistry and Materials Science
Chitosan
Chitosan - chemistry
Chitosan - pharmacology
Deacetylation
Fourier transforms
Fungi
Industrial wastes
Infrared spectroscopy
Onions
Original Article
Saccharomyces cerevisiae
Saccharomyces cerevisiae - chemistry
Sodium acetate
Staphylococcus aureus
Staphylococcus aureus - growth & development
Yeast
Yeasts
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Summary:Despite being widely available, Saccharomyces cerevisiae has not been widely explored for direct extraction of chitosan biopolymer for antimicrobial applications. In our study, S. cerevisiae from Baker’s yeast and Aspergillus niger from moldy onion extracts are studied as alternative sources of chitosan; and S cerevisiae chitosan tested for antimicrobial efficacy. The properties of S. cerevisiae chitosan are compared with moldy onion chitosan and shrimp chitosan extracted from shrimp shells. Chitosan extracted from S. cerevisiae is tested for antimicrobial efficacy against Staphylococcus Aureus. The maximum yields of fungal chitosan are 20.85 ± 0.35 mg/g dry S. cerevisiae biomass at 4th day using a culture broth containing sodium acetate, and 16.15 ± 0.95 mg/g dry A. niger biomass at 12th day. The degree of deacetylation (DD%) of the extracted fungal chitosan samples from S. cerevisiae and A. niger is found to be 63.4%, and 61.2% respectively, using Fourier Transform Infrared Spectroscopy. At a concentration of 2 g/L, S. cerevisiae chitosan shows the maximum inhibition zone diameter of 15.48 ± 0.07 mm. Baker’s yeast S cerevisiae biomass and A. niger from moldy onions has not been previously explored as a source of extractible fungal chitosan. This study gives insight that S. cerevisiae and A. niger from agricultural or industrial wastes could be a potential biomass source for production of the chitosan biopolymer. The S. cerevisiae chitosan displayed effective antimicrobial properties against S aureus , indicating the viablitiy of S cerevisae as a resource for extraction of high-quality chitosan.
ISSN:0273-2289
1559-0291
DOI:10.1007/s12010-021-03639-0