Activation of Hypothalamic AMP‐Activated Protein Kinase Ameliorates Metabolic Complications of Experimental Arthritis

Objective To investigate whether thermogenesis and the hypothalamus may be involved in the physiopathology of experimental arthritis (EA). Methods EA was induced in male Lewis rats by intradermal injection of Freund's complete adjuvant (CFA). Food intake, body weight, plasma cytokines, thermogr...

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Published in:Arthritis & rheumatology (Hoboken, N.J.) N.J.), 2022-02, Vol.74 (2), p.212-222
Main Authors: Seoane‐Collazo, Patricia, Rial‐Pensado, Eva, Estévez‐Salguero, Ánxela, Milbank, Edward, García‐Caballero, Lucía, Ríos, Marcos, Liñares‐Pose, Laura, Scotece, Morena, Gallego, Rosalía, Fernández‐Real, José Manuel, Nogueiras, Rubén, Diéguez, Carlos, Gualillo, Oreste, López, Miguel
Format: Article
Language:English
Subjects:
Adipose tissue
Adipose tissue (brown)
AMP-Activated Protein Kinases - physiology
Animals
Arthritis
Arthritis - complications
Arthritis - metabolism
Arthritis - physiopathology
Body fat
Body weight
Browning
Complications
Cytokines
Energy balance
Energy expenditure
Energy metabolism
Food intake
Gene expression
Homeostasis
Hypothalamus
Hypothalamus (ventromedial)
Hypothalamus - enzymology
Kinases
Male
Metabolism
Protein kinase
Proteins
Rats
Rats, Inbred Lew
Rheumatoid arthritis
Thermogenesis
Thermography
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Summary:Objective To investigate whether thermogenesis and the hypothalamus may be involved in the physiopathology of experimental arthritis (EA). Methods EA was induced in male Lewis rats by intradermal injection of Freund's complete adjuvant (CFA). Food intake, body weight, plasma cytokines, thermographic analysis, gene and protein expression of thermogenic markers in brown adipose tissue (BAT) and white adipose tissue (WAT), and hypothalamic AMP‐activated protein kinase (AMPK) were analyzed. Virogenetic activation of hypothalamic AMPK was performed. Results We first demonstrated that EA was associated with increased BAT thermogenesis and browning of subcutaneous WAT leading to elevated energy expenditure. Moreover, rats experiencing EA showed inhibition of hypothalamic AMPK, a canonical energy sensor modulating energy homeostasis at the central level. Notably, specific genetic activation of AMPK in the ventromedial nucleus of the hypothalamus (a key site modulating energy metabolism) reversed the effect of EA on energy balance, brown fat, and browning, as well as promoting amelioration of synovial inflammation in experimental arthritis. Conclusion Overall, these data indicate that EA promotes a central catabolic state that can be targeted and reversed by the activation of hypothalamic AMPK. This might provide new therapeutic alternatives to treat rheumatoid arthritis (RA)–associated metabolic comorbidities, improving the overall prognosis in patients with RA.
ISSN:2326-5191
2326-5205
DOI:10.1002/art.41950