Combined hyperosmolarity and inflammatory conditions in stressed human corneal epithelial cells and macrophages to evaluate osmoprotective agents as potential DED treatments

To develop an easy-to-perform combined model in human corneal epithelial cells (HCECs) and Balb/c mice macrophages J774.A1 (MP) for preliminary screening of potential ophthalmic therapeutic substances. HCECs were exposed to different osmolarities (350–500 mOsm/L) and MTT assay was employed for cell...

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Published in:Experimental eye research 2021-10, Vol.211, p.108723-108723, Article 108723
Main Authors: López-Cano, J.J., González-Cela-Casamayor, M.A., Andrés-Guerrero, V., Herrero-Vanrell, R., Benítez-Del-Castillo, J.M., Molina-Martínez, I.T.
Format: Article
Language:English
Subjects:
DED
Hyperosmolarity
Inflammation
Osmoprotectants
Osmoprotection model
Screening
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Summary:To develop an easy-to-perform combined model in human corneal epithelial cells (HCECs) and Balb/c mice macrophages J774.A1 (MP) for preliminary screening of potential ophthalmic therapeutic substances. HCECs were exposed to different osmolarities (350–500 mOsm/L) and MTT assay was employed for cell survival and flow cytometry to assess apoptosis-necrosis and relative cell size (RCS) distribution. Effectiveness of Betaine, L-Carnitine, Taurine at different concentrations (ranging from 20 mM to 200 mM) was studied. Also, mucoadhesive polymers such as Hyaluronic acid (HA) and Hydroxypropylmethylcellulose (HPMC) (0.4 and 0.8%) were evaluated. Cells were pre-incubated with the compounds (8h) and then exposed to hyperosmotic stress (470 mOsm/L) for 16h. Moreover, anti-inflammatory activity was performed in LPS-stimulated MP. Exposure to hyperosmotic solutions between 450 and 500 mOsm/L promoted the highest cell death after 16h exposures (p 
ISSN:0014-4835
1096-0007
DOI:10.1016/j.exer.2021.108723