Integration of a fiber-based cell culture and biosensing system for monitoring of multiple protein markers secreted from stem cells

We propose a new platform that can integrate three-dimensional cell culture scaffold and a surface-enhanced Raman spectroscopy (SERS)-based biosensor by stacking them to form a multilayer system, which would allow monitoring of the protein markers secreted from cultured stem cells without periodic c...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2021-12, Vol.193, p.113531-113531, Article 113531
Main Authors: Ko, Jaehan, Ham, Jeongwoo, Lee, Hwajung, Lee, Kangwon, Koh, Won-Gun
Format: Article
Language:English
Subjects:
Cell-secreted biomarker
Electrospun fibers
Integrated cell culture-biosensor platform
SERS
Stem cell differentiation
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Summary:We propose a new platform that can integrate three-dimensional cell culture scaffold and a surface-enhanced Raman spectroscopy (SERS)-based biosensor by stacking them to form a multilayer system, which would allow monitoring of the protein markers secreted from cultured stem cells without periodic cell and/or media collection. The cell culture scaffold supported the proliferation and osteogenic differentiation of adipose-derived mesenchymal stem cells (ADSCs). The SERS capture substrate detected protein markers in combination with SERS tag made with Au–Ag alloy nanoboxes. Incorporating the different Raman reporters into the SERS tag allowed easy identification of target proteins for multiplex assays. The resultant SERS-based immunoassay could detect the pg/mL levels of protein markers without crosstalk and interference. When one ADSC culture scaffold and multiple SERS capture substrates were integrated and incubated in differentiation culture media, our system was sufficiently sensitive to monitor time-dependent secretion of three different osteogenic protein markers from ADSCs during their osteogenic differentiation. Since the sensor and cell culture scaffold can be manipulated independently, various cell and biomarker combinations are possible to obtain relevant information regarding the actual state of the different types of cells. •Integration of 3D cell culture scaffolds and a SERS-based biosensor for multiplex detection of cellular metabolites.•Both cell culture scaffold and biosensing system were prepared from hydrogel-incorporated fiber matrix.•Integrated platform successfully monitored secretion of multiple biomarkers during osteogenesis of ADSCS.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2021.113531