Ascaris lumbricoides and ticks associated with sensitization to galactose α1,3-galactose and elicitation of the alpha-gal syndrome

IgE to galactose alpha-1,3 galactose (alpha-gal) causes alpha-gal syndrome (delayed anaphylaxis after ingestion of mammalian meat). Development of sensitization has been attributed to tick bites; however, the possible role of other parasites has not been well studied. Our aims were to assess the pre...

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Published in:Journal of allergy and clinical immunology 2022-02, Vol.149 (2), p.698-707.e3
Main Authors: Murangi, Tatenda, Prakash, Prema, Moreira, Bernardo Pereira, Basera, Wisdom, Botha, Maresa, Cunningham, Stephen, Facey-Thomas, Heidi, Halajian, Ali, Joshi, Lokesh, Ramjith, Jordache, Falcone, Franco H., Horsnell, William, Levin, Michael E.
Format: Article
Language:English
Subjects:
Alpha-gal
Amblyomma hebraeum
anaphylaxis
Animals
Antigens, Helminth - immunology
Ascaris lumbricoides
Ascaris lumbricoides - immunology
Cells, Cultured
Disaccharides - analysis
food allergy
Food Hypersensitivity - etiology
galactose-alpha-1,3-galactose
helminths
Humans
Immunoglobulin E - immunology
meat allergy
Rats
red meat allergy
Rhipicephalus evertsi
ticks
Ticks - immunology
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Summary:IgE to galactose alpha-1,3 galactose (alpha-gal) causes alpha-gal syndrome (delayed anaphylaxis after ingestion of mammalian meat). Development of sensitization has been attributed to tick bites; however, the possible role of other parasites has not been well studied. Our aims were to assess the presence, relative abundances, and site of localization of alpha-gal–containing proteins in common ectoparasites and endoparasites endemic in an area of high prevalence of alpha-gal syndrome, as well as to investigate the ability of ascaris antigens to elicit a reaction in a humanized rat basophil in vitro sensitization model. Levels of total IgE, Ascaris-specific IgE, and alpha-gal IgE were measured in sera from patients with challenge-proven alpha-gal syndrome and from controls without allergy. The presence, concentration, and localization of alpha-gal in parasites were assessed by ELISA, Western blotting, and immunohistochemistry. The ability of Ascaris lumbricoides antigen to elicit IgE-dependent reactivity was demonstrated by using the RS-ATL8 basophil reporter system. Alpha-gal IgE level correlated with A lumbricoides–specific IgE level. Alpha-gal protein at 70 to 130 kDa was detected in A lumbricoides at concentrations higher than those found in Rhipicephalus evertsi and Amblyomma hebraeum ticks. Immunohistochemistry was used to localize alpha-gal in tick salivary acini and the helminth gut. Non–alpha-gal–containing A lumbricoides antigens activated RS-ATL8 basophils primed with serum from subjects with alpha-gal syndrome. We demonstrated the presence, relative abundances, and site of localization of alpha-gal–containing proteins in parasites. The activation of RS-ATL8 IgE reporter cells primed with serum from subjects with alpha-gal syndrome on exposure to non–alpha-gal–containing A lumbricoides proteins indicates a possible role of exposure to A lumbricoides in alpha-gal sensitization and clinical reactivity.
ISSN:0091-6749
1097-6825
DOI:10.1016/j.jaci.2021.07.018