Multifunctional T cell response in active pulmonary tuberculosis patients

•·APTB patients have decreased T cells but its function is enhanced.•·Multifunctional CD4+ T cells in APTB patients can migrate to the lungs.•·Multifunctional T cells may be an indicator for monitoring disease treatment. Tuberculosis still threatens human health. We aimed to investigate the T cell i...

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Bibliographic Details
Published in:International immunopharmacology 2021-10, Vol.99, p.107898-107898, Article 107898
Main Authors: Qin, Shuang, Chen, Ruiqi, Jiang, Yujie, Zhu, Hengyue, Chen, Lijiang, Chen, Yanfan, Shen, Mo, Lin, Xiangyang
Format: Article
Language:English
Subjects:
Adult
Aged
Blood
CC chemokine receptors
CCR7 protein
CD4 antigen
CD4-Positive T-Lymphocytes - immunology
CD45RA antigen
CD8 antigen
CD8-Positive T-Lymphocytes - immunology
Cell activation
Cell differentiation
Chemokines
CXCR3 protein
Cytokines - blood
Female
Granzyme B
Granzymes - immunology
Health risks
Humans
Immune
Immune status
IP-10 protein
Leukocyte migration
Lymphocyte Count
Lymphocytes
Lymphocytes T
Male
Medical treatment
Middle Aged
Multifunctional T cells
Patients
Perforin
Perforin - immunology
Peripheral blood
Phenotypes
Pleural fluid
T-Lymphocyte Subsets - immunology
Tuberculosis
Tuberculosis, Pulmonary - blood
Tuberculosis, Pulmonary - immunology
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Summary:•·APTB patients have decreased T cells but its function is enhanced.•·Multifunctional CD4+ T cells in APTB patients can migrate to the lungs.•·Multifunctional T cells may be an indicator for monitoring disease treatment. Tuberculosis still threatens human health. We aimed to investigate the T cell immune status and the role of multifunctional T cells in pulmonary tuberculosis patients. Thirty active pulmonary tuberculosis (APTB) patients, 30 latent tuberculosis infection (LTBI) patients, 25 cured pulmonary tuberculosis (CPTB) patients and 25 healthy controls (HCs) enrolled in this study. Flow cytometer for detecting T cell phenotype and function. CBA Flex Set was used to measure chemokine. Compared with HCs and LTBI patients, APTB patients had fewer CD4+ T and CD8+ T cells, but the expression of granzyme A, granzyme B and perforin on CD8+ T cells increased. Compared to LTBI and CPTB patients, Mycobacterium tuberculosis-specific CD8+ T cells in APTB patients appeared to be more differentiated CD45RA-CCR7- cells, and there were more multifunctional CD4+ T and CD8+ T cells. Importantly, the frequency of multifunctional CD4+ T cells in the pleural fluid of APTB patients was higher than that of peripheral blood. And the proportion of multifunctional CD4+ T cells expressing the migration receptor CXCR3 in the peripheral blood of APTB patients decreased, while the concentrations of its ligands, chemokine MIG, IP-10 and I-TAC increased significantly in plasma, especially in pleural fluid. Decreased T lymphocytes in APTB patients may cause compensatory activation of CD8+ T cells. Multifunctional CD4+ T cells in peripheral blood could migrate to the lungs under the action of CXCR3 and associated chemokine. Multifunctional CD4+ T cells and Multifunctional CD8+ T cells were of great significance in monitoring disease treatment.
ISSN:1567-5769
1878-1705
DOI:10.1016/j.intimp.2021.107898