Phenotypic screening system using three-dimensional (3D) culture models for natural product screening

Recent progress in three-dimensional (3D) cell culture systems has attracted much attention in the fields of basic life science and drug development. Newly established methods include 3D co-culture, spheroid culture, and organoid culture; these methods enable more human tissue-like culture and have...

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Bibliographic Details
Published in:Journal of antibiotics 2021-10, Vol.74 (10), p.660-666
Main Authors: Suenaga, Hikaru, Kagaya, Noritaka, Kawada, Manabu, Tatsuda, Daisuke, Sato, Toshiro, Shin-Ya, Kazuo
Format: Article
Language:English
Subjects:
Alzheimer's disease
Animals
Antibiotics
Biological Products - pharmacology
Cell culture
Cell Culture Techniques
Cell Line
Coculture Techniques
Cytokines
Drug development
Fibroblasts
Gene expression
Human tissues
Humans
Hypoxia
Medical prognosis
Medical research
Natural products
Organoids
Organoids - drug effects
Pancreatic cancer
Physiology
Prostate cancer
Screening
Self-assembly
Spheroids, Cellular - drug effects
Stem cells
Three dimensional models
Tissue culture
Tumors
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Summary:Recent progress in three-dimensional (3D) cell culture systems has attracted much attention in the fields of basic life science and drug development. Newly established methods include 3D co-culture, spheroid culture, and organoid culture; these methods enable more human tissue-like culture and have largely replaced traditional two-dimensional (2D) monolayer culture. By combining 3D culture methods with high-content imaging analysis, it is possible to obtain diverse and convincing data even during initial screening (which requires rapid and easy operating procedures). Until recently, 3D culture methods were considered expensive, time-consuming, complex, and unstable. However, by exploiting the self-assembling nature of cells and adding several technical improvements, we have developed several phenotypic screenings aimed at discovering anticancer compounds.
ISSN:0021-8820
1881-1469
DOI:10.1038/s41429-021-00457-8