RtNAC100 involved in the regulation of ROS, Na+ accumulation and induced salt-related PCD through MeJA signal pathways in recretohalophyte Reaumuria trigyna

•Reaumuria trigyna is a halophyte useful for uncovering the mechanism of plant salt tolerance.•Methyl jasmonate upregulated RtNAC100 expression.•RtNAC100 interacted with RtRbohE/SAG12, accelerating salt-induced programmed cell death.•RtNAC100 promoted reactive oxygen species, Ca2+, and Na+ accumulat...

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Bibliographic Details
Published in:Plant science (Limerick) 2021-09, Vol.310, p.110976-110976, Article 110976
Main Authors: Ma, Binjie, Liu, Xiaofei, Guo, Shuyu, Xie, Xinlei, Zhang, Jie, Wang, Jianye, Zheng, Linlin, Wang, Yingchun
Format: Article
Language:English
Subjects:
PCD
Reaumuria trigyna
ROS
RtNAC100
Salt stress
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Summary:•Reaumuria trigyna is a halophyte useful for uncovering the mechanism of plant salt tolerance.•Methyl jasmonate upregulated RtNAC100 expression.•RtNAC100 interacted with RtRbohE/SAG12, accelerating salt-induced programmed cell death.•RtNAC100 promoted reactive oxygen species, Ca2+, and Na+ accumulation and increased the Na+/K+ ratio. NAM, ATAF1/2, and CUC2 (NAC) proteins regulate plant responses to salt stress. However, the molecular mechanisms by which NAC proteins regulate salt-induced programmed cell death (PCD) are unclear. We identified 56 NAC genes, 35 of which had complete open reading frames with complete NAM domain, in the R. trigyna transcriptome. Salt stress and methyl jasmonate (MeJA) mediated PCD-induced leaf senescence in R. trigyna seedlings. Salt stress accelerated endogenous JA biosynthesis, upregulating RtNAC100 expression. This promoted salt-induced leaf senescence in R. trigyna by regulating RtRbohE and RtSAG12/20 and enhancing ROS accumulation. Transgenic assays showed that RtNAC100 overexpression aggravated salt-induced PCD in transgenic lines by promoting ROS and Na+ accumulation, ROS-Ca2+ hub activation, and PCD-related gene expression. Therefore, RtNAC100 induces PCD via the MeJA signaling pathway in R. trigyna under salt stress.
ISSN:0168-9452
1873-2259
DOI:10.1016/j.plantsci.2021.110976