Apoptosis Induction in Murine Melanoma (B16F10) Cells by Mannosylerythritol Lipids-B; a Glycolipid Biosurfactant with Antitumoral Activities

Mannosylerythritol lipids have drawn attention to cosmetic and pharmaceutical industries due to their non-toxicity and excellent biological interactions with human skin, particularly with the deepest epidermal layer. Lamellar liquid crystal structure, formed by MEL-B, is an interesting feature due t...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 2021-11, Vol.193 (11), p.3855-3866
Main Authors: Feuser, P. E., Coelho, A. L. S., de Melo, M. E., Scussel, R., Carciofi, B. A. M., Machado-de-Ávila, R. A., de Oliveira, D., de Andrade, C. J.
Format: Article
Language:English
Subjects:
Animals
Anticancer properties
Antineoplastic Agents - chemistry
Antineoplastic Agents - pharmacology
Apoptosis
Apoptosis - drug effects
Biochemistry
Biotechnology
Cell membranes
Cell signaling
Cell viability
Chemistry
Chemistry and Materials Science
Crystal structure
Cytotoxicity
Cytotoxins - chemistry
Cytotoxins - pharmacology
Deregulation
Erythrocytes
Fibroblasts
Fluorescence
Fluorescence microscopy
Glycolipids - chemistry
Glycolipids - pharmacology
Lamellar structure
Lipids
Liquid crystals
Melanoma
Melanoma, Experimental - drug therapy
Melanoma, Experimental - metabolism
Mice
Pharmaceutical industry
Reactive oxygen species
Short Communication
Stratum corneum
Surface-Active Agents - chemistry
Surface-Active Agents - pharmacology
Toxicity
Tumor cells
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Description
Summary:Mannosylerythritol lipids have drawn attention to cosmetic and pharmaceutical industries due to their non-toxicity and excellent biological interactions with human skin, particularly with the deepest epidermal layer. Lamellar liquid crystal structure, formed by MEL-B, is an interesting feature due to its similarity to the stratum corneum molecular arrangement and cell signaling events involved in the deregulation of the cancerous cell membrane. Thus, this work aimed to evaluate the cytotoxicity of commercial mannosylerythritol lipids-B in murine melanoma, fibroblast, and human erythrocytes cells. Cytotoxic effect was more pronounced on the tumor cells from 20 µg/mL, reducing cell viability by 65%, whereas fibroblast and human erythrocytes cells were more resistant to glycolipid treatment. Fluorescence microscopy and flow cytometer proved that mannosylerythritol lipids-B is an apoptosis inducer in tumor cells related to reactive oxygen species generation.
ISSN:0273-2289
1559-0291
DOI:10.1007/s12010-021-03620-x