Differentiable model-based adaptive optics for two-photon microscopy

Aberrations limit scanning fluorescence microscopy when imaging in scattering materials such as biological tissue. Model-based approaches for adaptive optics take advantage of a computational model of the optical setup. Such models can be combined with the optimization techniques of machine learning...

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Bibliographic Details
Published in:Optics express 2021-07, Vol.29 (14), p.21418-21427
Main Authors: Vishniakou, Ivan, Seelig, Johannes D.
Format: Article
Language:English
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Summary:Aberrations limit scanning fluorescence microscopy when imaging in scattering materials such as biological tissue. Model-based approaches for adaptive optics take advantage of a computational model of the optical setup. Such models can be combined with the optimization techniques of machine learning frameworks to find aberration corrections, as was demonstrated for focusing a laser beam through aberrations onto a camera [ Opt. Express 28 26436 ( 26436 ) 10.1364/OE.403487 ]. Here, we extend this approach to two-photon scanning microscopy. The developed sensorless technique finds corrections for aberrations in scattering samples and will be useful for a range of imaging application, for example in brain tissue.
ISSN:1094-4087
1094-4087
DOI:10.1364/OE.424344