Addition of EP2 agonists to an FP agonist additively and synergistically modulates adipogenesis and the physical properties of 3D 3T3-L1 sphenoids

•Addition of EP2 agonists to FP agonist modulate adipogenesis of the 2D and 3D cultured 3T3-L1 cells.•EP2 agonists synergistically altered physical properties of the 3T3-L1 sphenoid with FP agonist.•3D culture was useful technique for evaluation of adipocytes by PG derivatives. The additive effects...

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Published in:Prostaglandins, leukotrienes and essential fatty acids leukotrienes and essential fatty acids, 2021-08, Vol.171, p.102315-102315, Article 102315
Main Authors: Ida, Yosuke, Watanabe, Megumi, Umetsu, Araya, Ohguro, Hiroshi, Hikage, Fumihito
Format: Article
Language:English
Subjects:
3T3-L1
3T3-L1 Cells
Adipogenesis - drug effects
Adipogenesis - genetics
Alprostadil - analogs & derivatives
Alprostadil - pharmacology
Animals
Bimatoprost - pharmacology
Cell Culture Techniques, Three Dimensional
Collagen Type I - drug effects
Collagen Type I - genetics
Collagen Type I - metabolism
Collagen Type IV - drug effects
Collagen Type IV - genetics
Collagen Type IV - metabolism
Collagen Type VI - drug effects
Collagen Type VI - genetics
Collagen Type VI - metabolism
Drug Synergism
EP2 agonist
Extracellular Matrix - drug effects
Extracellular Matrix - metabolism
Fatty Acid-Binding Proteins - drug effects
Fatty Acid-Binding Proteins - genetics
Fatty Acid-Binding Proteins - metabolism
Fibronectins - drug effects
Fibronectins - genetics
Fibronectins - metabolism
FPagonist
Glycine - analogs & derivatives
Glycine - pharmacology
Leptin - genetics
Leptin - metabolism
Mice
PPAR gamma - drug effects
PPAR gamma - genetics
PPAR gamma - metabolism
Pyrazoles - pharmacology
Pyridines - pharmacology
Receptors, Prostaglandin - agonists
Receptors, Prostaglandin E, EP2 Subtype - agonists
Spheroids, Cellular - drug effects
Spheroids, Cellular - metabolism
Three-dimension culture
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Summary:•Addition of EP2 agonists to FP agonist modulate adipogenesis of the 2D and 3D cultured 3T3-L1 cells.•EP2 agonists synergistically altered physical properties of the 3T3-L1 sphenoid with FP agonist.•3D culture was useful technique for evaluation of adipocytes by PG derivatives. The additive effects of prostaglandin (PG)-EP2 agonists on a PG-FP agonist toward adipogenesis in two- or three-dimension (2D or 3D) cultures of 3T3-L1 cells was examined by lipid staining, the mRNA expression of adipogenesis related genes, and extracellular matrixes (ECMs) including collagen molecules (Col) -1, -4 and -6, and fibronectin (Fn), and the sizes and physical properties of 3D sphenoids, as measured by a micro-squeezer. The results indicate that adipogenesis induced 1) an enlargement in the sizes of 3D sphenoids, 2) a substantial enhancement in lipid staining, the expression of the PParγ, Ap2 and Leptin genes, and 3) a significant decrease in the stiffness of the 3D sphenoids. These effects were inhibited by bimatoprost acid (BIM-A), but 4) adipogenesis induced significant down-regulation of Col1 and Fn, and the significant up-regulation of the Col4 and Col6 genes were unchanged by BIM-A. On the addition of an EP2 agonist, such as omidenepag (OMD) or butaprost (Buta), to BIM-A, 1) the sizes of the 3D sphenoids were further decreased, 2) lipid staining was decreased (2D; OMD, 3D; Buta) 3) the stiffness of the 3D sphenoids was increased by Buta, 4) the expression of PParγ was up-regulated (2D; Buta) or unchanged (3D), the expression of Ap2 was down-regulated (2D; OMD) or up-regulated (3D; Buta), and the expression of Leptin was increased (2D), 5) the expression of all four (OMD) or all except Col4 (buta) in 2D, and Col1and Col4 (OMD) in 3D were up-regulated. These collective findings indicate that the addition of an EP2 agonist, OMD or Buta significantly modulated the BIM-A induced suppression of adipogenesis as well as physical properties of 2D and 3D cultured 3T3-L1 cells in different manners.
ISSN:0952-3278
1532-2823
DOI:10.1016/j.plefa.2021.102315