Measurement of histone replacement dynamics with genetically encoded exchange timers in yeast

Histone exchange between histones carrying position-specific marks and histones bearing general marks is important for gene regulation, but understanding of histone exchange remains incomplete. To overcome the poor time resolution of conventional pulse-chase histone labeling, we present a geneticall...

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Bibliographic Details
Published in:Nature biotechnology 2021-11, Vol.39 (11), p.1434-1443
Main Authors: Yaakov, Gilad, Jonas, Felix, Barkai, Naama
Format: Article
Language:English
Subjects:
Analysis
Cell cycle
DNA methylation
Enzymes
Epigenetics
Exchanging
Foreign exchange rates
Gene regulation
Genes
Genetic aspects
Genetic code
Genomes
Genomics
Histones
Identification and classification
In vivo methods and tests
Labeling
Localization
Methods
Nucleosomes
Physiological aspects
Properties
Sensors
Timing devices
Transcription
Yeast
Yeast fungi
Yeasts
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Summary:Histone exchange between histones carrying position-specific marks and histones bearing general marks is important for gene regulation, but understanding of histone exchange remains incomplete. To overcome the poor time resolution of conventional pulse-chase histone labeling, we present a genetically encoded histone exchange timer sensitive to the duration that two tagged histone subunits co-reside at an individual genomic locus. We apply these sensors to map genome-wide patterns of histone exchange in yeast using single samples. Comparing H3 exchange in cycling and G1-arrested cells suggests that replication-independent H3 exchange occurs at several hundred nucleosomes (
ISSN:1087-0156
1546-1696
DOI:10.1038/s41587-021-00959-8