X‐ray micro‐computed tomography in the assessment of penile cavernous fibrosis in a rabbit castration model

Background Current assessment methods of penile cavernous fibrosis in animal models have limitations due to the inability to provide complex and volume analysis of fibrotic alterations. Objective The aim was to evaluate micro‐computed tomography for assessment of cavernous fibrosis and compare it wi...

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Bibliographic Details
Published in:Andrology (Oxford) 2021-09, Vol.9 (5), p.1467-1480
Main Authors: Kogan, Mikhail I., Popov, Igor V., Kirichenko, Evgeniya Y., Mitrin, Boris I., Sadyrin, Evgeniy V., Kulaeva, Elizaveta D., Popov, Ilya V., Kulba, Sergey N., Logvinov, Alexander K., Akimenko, Marina A., Pasechnik, Dmitry G., Tkachev, Sergey Yu, Karnaukhov, Nikolay S., Lapteva, Tatyana O., Sukhar, Irina A., Maksimov, Alexey Yu, Ermakov, Alexey M.
Format: Article
Language:English
Subjects:
3D X‐ray microscopy
animal model
Animals
cavernous fibrosis
Connective tissue
corpora cavernosa
Disease Models, Animal
gray value analysis
Imaging, Three-Dimensional - methods
Male
micro‐computed tomography
Muscle, Smooth - diagnostic imaging
Muscle, Smooth - metabolism
Orchiectomy
Penile Induration - chemically induced
Penile Induration - diagnostic imaging
Penile Induration - pathology
Penis
Penis - diagnostic imaging
Penis - metabolism
Penis - pathology
Rabbits
radiodensity
Real-Time Polymerase Chain Reaction
Tomography
Transforming Growth Factor beta1 - metabolism
Value analysis
X-Ray Microtomography
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Summary:Background Current assessment methods of penile cavernous fibrosis in animal models have limitations due to the inability to provide complex and volume analysis of fibrotic alterations. Objective The aim was to evaluate micro‐computed tomography for assessment of cavernous fibrosis and compare it with histological, histochemical, immunohistochemical, and RT‐PCR analysis. Materials and methods A controlled trial was performed involving 25 New Zealand male rabbits with induced testosterone deficiency by orchidectomy. Penile samples were obtained before and after 7, 14, 21, and 84 days from orchidectomy. We consistently performed (a) gray value analysis of corpora cavernosa 3D models reconstructed after micro‐computed tomography, (b) morphometry of smooth muscles/connective tissue ratio, collagen type I/III ratio, and area of TGF‐beta‐1 expression in corpora cavernosa, and (c) RT‐PCR of TGF‐beta‐1 expression. Results Micro‐computed tomography allowed visualization of penile structures at a resolution comparable to light microscopy. Gray values of corpora cavernosa decreased from 1673 (1512–1773) on the initial day to 1184 (1089–1232) on the 21st day (p 
ISSN:2047-2919
2047-2927
DOI:10.1111/andr.13077