Peroxidase-mimetic activity of FeOCl nanosheets for the colorimetric determination of glutathione and cysteine

For the first time the enzyme mimic activity of iron oxychloride (FeOCl) nanosheets has been studied. The intrinsic peroxidase-mimetic activity of the nanosheets in the presence of H 2 O 2 was approved by the efficient oxidation of tetramethylbenzidine (TMB). The Michaelis–Menten constant of the nan...

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Bibliographic Details
Published in:Mikrochimica acta (1966) 2021-07, Vol.188 (7), p.239-239, Article 239
Main Authors: Mohammadpour, Zahra, Malekian Jebeli, Fatemeh, Ghasemzadeh, Sahel
Format: Article
Language:English
Subjects:
Analytical Chemistry
Benzidines - chemistry
Biomolecules
Biosensors
Catalysis
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Chromogenic Compounds - chemistry
Colorimetry
Colorimetry - methods
Cysteine
Cysteine - blood
Cysteine - chemistry
Cystine
Glutathione
Glutathione - blood
Glutathione - chemistry
Humans
Hydrogen peroxide
Hydrogen Peroxide - chemistry
Iron Compounds - chemistry
Limit of Detection
Microengineering
Nanochemistry
Nanosheets
Nanostructures - chemistry
Nanotechnology
Original Paper
Oxidation
Oxidation-Reduction
Peroxidase
Selectivity
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Summary:For the first time the enzyme mimic activity of iron oxychloride (FeOCl) nanosheets has been studied. The intrinsic peroxidase-mimetic activity of the nanosheets in the presence of H 2 O 2 was approved by the efficient oxidation of tetramethylbenzidine (TMB). The Michaelis–Menten constant of the nanosheets toward TMB was about six times lower than that of natural horseradish peroxidase. The superiority of the nanosheets’ catalytic property ascribes to their H 2 O 2 activation ability. Based on the inhibition of the nanozymes’ catalytic reaction, an assay was developed for the quantitative measurement of glutathione (GSH) and cysteine (Cys). The linear range for both biomolecules was over the range of 3–33 μM. The LOD values (3σ/slope) for GSH and Cys were 2.23 μM and 2.76 μM, respectively. Importantly, we succeeded in colorimetric discrimination of GSH and Cys kinetically. We achieved high selectivity toward GSH and Cys. This work extends the feasibility of using FeOCl as nanozymes to construct biosensors, colorimetric probes for medical diagnosis, and nanozyme-based cancer therapy. Graphical abstract
ISSN:0026-3672
1436-5073
DOI:10.1007/s00604-021-04903-0