Survey of Drought-Associated TAWRKY2-D1 Gene Diversity in Bread Wheat and Wheat Relatives

Recent advances in plant genomics revealed numerous factors related to drought tolerance, including a family of WRKY transcription factors. The aim of this study was to evaluate polymorphism of the TaWRKY2 - D1 across a range of bread wheat cultivars, interspecific hybrids, and wild wheat relatives...

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Bibliographic Details
Published in:Molecular biotechnology 2021-10, Vol.63 (10), p.953-962
Main Authors: Lakhneko, Olha, Stepanenko, Anton, Kuzminskiy, Yevhen, Borisjuk, Nikolai, Morgun, Bogdan
Format: Article
Language:English
Subjects:
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Biochemistry
Biological Techniques
Biotechnology
Bread
Bread - classification
Cell Biology
Chemistry
Chemistry and Materials Science
Cultivars
Deoxyribonucleic acid
DNA
Drought resistance
Droughts
Exons
Genetic diversity
Germplasm
Heterogeneity
Human Genetics
Hybrids
Introns
Markers
Molecular Sequence Annotation
Original Paper
Plant Breeding
Plant Proteins - genetics
Polymorphism
Polymorphism, Genetic
Promoter Regions, Genetic
Protein Science
Sequence Analysis, DNA - methods
Transcription factors
Transcription Factors - genetics
Triticum
Triticum - classification
Triticum - genetics
Triticum - growth & development
Wheat
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Summary:Recent advances in plant genomics revealed numerous factors related to drought tolerance, including a family of WRKY transcription factors. The aim of this study was to evaluate polymorphism of the TaWRKY2 - D1 across a range of bread wheat cultivars, interspecific hybrids, and wild wheat relatives within the Triticum genus as a potential molecular target for marker-assistant selection. The initial sequencing of the TaWRKY2 - D1 gene in six Ukrainian commercial cultivars detected some sequence variations along the ~ 1.8 kb of gene promoter and the followed coding region composed of four exons and three introns. Based on the gained sequence information, five sets of primers covering different gene regions were designed to annotate the TaWRKY2 - D1 genetic diversity in 202 wheat cultivars, including 77 accessions from the CIMMYT collection, 72 commercial varieties cultivated in Ukraine, and 53 hybrids and wild wheat species. The combination of developed DNA markers enabled effective and reproducible annotation of cultivars genetic diversity. The primers set targeting introns adjusted to the gene’s exon 3, turned out to be the most informative for screening heterogeneity of the TaWRKY2-D1 . The developed molecular markers represent effective, informative means for selecting drought tolerance germplasm donors to promote wheat breeding programs.
ISSN:1073-6085
1559-0305
DOI:10.1007/s12033-021-00350-7