On‐chip determination of tissue‐specific metastatic potential of breast cancer cells

Metastasis is one of the major obstacles for breast cancer patients. Limitations of current models demand the development of custom platforms to predict metastatic potential and homing choices of cancer cells. Here, two organ‐on‐chip platforms, invasion/chemotaxis (IC‐chip) and extravasation (EX‐chi...

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Bibliographic Details
Published in:Biotechnology and bioengineering 2021-10, Vol.118 (10), p.3799-3810
Main Authors: Firatligil‐Yildirir, Burcu, Bati‐Ayaz, Gizem, Tahmaz, Ismail, Bilgen, Muge, Pesen‐Okvur, Devrim, Yalcin‐Ozuysal, Ozden
Format: Article
Language:English
Subjects:
Biochips
Breast cancer
Breast Neoplasms - metabolism
Breast Neoplasms - pathology
Cell Movement
Chemotaxis
Extravasation
Female
Human Umbilical Vein Endothelial Cells
Humans
In vivo methods and tests
Integrated circuits
invasion
Invasiveness
Lab-On-A-Chip Devices
lab‐on‐a‐chip
Liver
Lung cancer
Lungs
MCF-7 Cells
Metastases
Metastasis
Microenvironments
Models, Biological
Neoplasm Invasiveness
Neoplasm Metastasis
Phenotypes
Platforms
Tissues
Tumor Microenvironment
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Summary:Metastasis is one of the major obstacles for breast cancer patients. Limitations of current models demand the development of custom platforms to predict metastatic potential and homing choices of cancer cells. Here, two organ‐on‐chip platforms, invasion/chemotaxis (IC‐chip) and extravasation (EX‐chip) were used for the quantitative assessment of invasion and extravasation towards specific tissues. Lung, liver and breast microenvironments were simulated in the chips using tissue‐specific cells embedded in matrigel. In the IC‐chip, invasive MDA‐MB‐231, but not noninvasive MCF‐7 breast cancer cells invaded into lung and liver microenvironments. In the EX‐chip, MDA‐MB‐231 cells extravasated more into the lung compared to the liver and breast microenvironments. In addition, lung‐specific MDA‐MB‐231 clone invaded and extravasated into the lung microenvironment more efficiently than the bone‐specific clone. Both invasion/chemotaxis and extravasation results were in agreement with published clinical data. Collectively, our results show that IC‐chip and EX‐chip, simulating tissue‐specific microenvironments, can distinguish different in vivo metastatic phenotypes, in vitro. Determination of tissue‐specific metastatic potential of breast cancer cells is expected to improve diagnosis and help select the ideal therapy. Two organ‐on‐chip platforms, invasion/chemotaxis (IC‐chip) and extravasation (EX‐chip) were developed for the quantitative assessment of invasion and extravasation of breast cancer cells towards specific tissues. IC‐chip and EX‐chip, simulating lung‐, liver‐ and breast‐specific microenvironments in vitro, can distinguish different in vivo metastatic phenotypes.
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.27855