Genome-wide analysis of ARF transcription factors reveals HcARF5 expression profile associated with the biosynthesis of β-ocimene synthase in Hedychium coronarium

Key message Herein, 37 ARF genes were identified and analyzed in Hedychium coronarium and HcARF5 showed a potential role in the regulation of HcTPS3. Auxin is an important plant hormone, implicated in various aspects of plant growth and development processes especially in the biosynthesis of various...

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Bibliographic Details
Published in:Plant cell reports 2021-07, Vol.40 (7), p.1269-1284
Main Authors: Abbas, Farhat, Ke, Yanguo, Zhou, Yiwei, Yu, Yunyi, Waseem, Muhammad, Ashraf, Umair, Li, Xinyue, Yu, Rangcai, Fan, Yanping
Format: Article
Language:English
Subjects:
Acyclic Monoterpenes - metabolism
Alkenes - metabolism
Alkyl and Aryl Transferases - genetics
Alkyl and Aryl Transferases - metabolism
Assaying
Biomedical and Life Sciences
Biosynthesis
Biotechnology
Cell Biology
Complementation
Emission analysis
Flowering
Flowers
Flowers - genetics
Flowers - growth & development
Fluorescence
Gene expression
Gene Expression Regulation, Plant - drug effects
Gene silencing
Genes
Genome, Plant
Genomes
Hedychium coronarium
Life Sciences
Metabolites
MicroRNAs
Ocimene
Original Article
Phylogeny
Plant Biochemistry
Plant growth
Plant Growth Regulators - pharmacology
Plant Proteins - genetics
Plant Proteins - metabolism
Plant Sciences
Plants (botany)
Regulatory mechanisms (biology)
Regulatory Sequences, Nucleic Acid
Secondary metabolites
Terpene synthase
Transcription activation
Transcription factors
Transcription Factors - genetics
Transcription Factors - metabolism
Transcriptomes
Two-Hybrid System Techniques
Volatile compounds
Yeast
Yeasts
Zingiberaceae - drug effects
Zingiberaceae - genetics
Zingiberaceae - metabolism
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Summary:Key message Herein, 37 ARF genes were identified and analyzed in Hedychium coronarium and HcARF5 showed a potential role in the regulation of HcTPS3. Auxin is an important plant hormone, implicated in various aspects of plant growth and development processes especially in the biosynthesis of various secondary metabolites. Auxin response factors (ARF) belong to the transcription factors (TFs) gene family and play a crucial role in transcriptional activation/repression of auxin-responsive genes by directly binding to their promoter region. Nevertheless, whether ARF genes are involved in the regulatory mechanism of volatile compounds in flowering plants is largely unknown. β-ocimene is a key floral volatile compound synthesized by terpene synthase 3 ( HcTPS3 ) in Hedychium coronarium . A comprehensive analysis of H . coronarium genome reveals 37 candidate ARF genes in the whole genome. Tissue-specific expression patterns of HcARFs family members were assessed using available transcriptome data. Among them, HcARF5 showed a higher expression level in flowers, and significantly correlated with the key structural β-ocimene synthesis gene ( HcTPS3 ). Furthermore, transcript levels of both genes were associated with the flower development. Under hormone treatments, the response of HcARF5 and HcTPS3, and the emission level of β-ocimene contents were evaluated. Subcellular and transcriptional activity assay showed that HcARF5 localizes to the nucleus and possesses transcriptional activity. Yeast one-hybrid (Y1H) and dual-luciferase assays revealed that HcARF5 directly regulates the transcriptional activity of HcTPS3 . Yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays showed that HcARF5 interacts with scent-related HcIAA4, HcIAA6, and HcMYB1 in vivo. Overall, these results indicate that HcARF5 is potentially involved in the regulation of β-ocimene synthesis in H. coronarium .
ISSN:0721-7714
1432-203X
DOI:10.1007/s00299-021-02709-1