Metallococcus carri gen. nov., sp. nov., a novel member of the family Dermacoccaceae isolated from an automotive air conditioning system

Strain designated DB0510 T was isolated from an automobile evaporator core collected in Korea. Cells are gram-stain-positive, aerobic, and coccoid. The strain grew at 15–45 ℃, pH 5.0–8.5 and 0–8.0% (w/v) NaCl. Growth occurs on R2A, trypticase soy agar, Luria–Bertani agar, and nutrient agar. Phylogen...

Full description

Saved in:
Bibliographic Details
Published in:Archives of microbiology 2021-09, Vol.203 (7), p.4073-4079
Main Authors: Lim, Jong Sung, Lee, Hyosun, Son, Jigwan, Kim, Song-Gun, Kim, Dong-Uk, Ka, Jong-Ok
Format: Article
Language:English
Subjects:
Air conditioning
Alanine
Amino acids
Aspartic acid
Biochemistry
Biomedical and Life Sciences
Biotechnology
Cell Biology
Deoxyribonucleic acid
Dermacoccaceae
Diphosphatidylglycerol
DNA
Ecology
Evaporators
Fatty acids
Genomes
Glutamic acid
Glycine
Gram-positive cocci
Life Sciences
Lipids
Lysine
Menaquinones
Metallococcus carri
Microbial Ecology
Microbiology
New species
Novel Prokaryotic Taxa
Nucleotide sequence
Nucleotides
Original Paper
Peptidoglycans
Phosphatidylglycerol
Phosphatidylinositol
Phylogeny
rRNA 16S
Serine
Sodium chloride
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Strain designated DB0510 T was isolated from an automobile evaporator core collected in Korea. Cells are gram-stain-positive, aerobic, and coccoid. The strain grew at 15–45 ℃, pH 5.0–8.5 and 0–8.0% (w/v) NaCl. Growth occurs on R2A, trypticase soy agar, Luria–Bertani agar, and nutrient agar. Phylogenetic analysis showed that the strain belongs to the family Dermacoccaceae and strain DB0510 T was distinctly separated from validly named genera of this family. Signature nucleotides in 16S rRNA gene sequence revealed that the strain contained the Dermacoccaceae family-specific 16S rRNA signature nucleotides patterns. The major fatty acids were C 17:0 and C 17:1 cis-9. The only menaquinone was MK-8 (H 4 ). The polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, and two unidentified lipids. The diagnostic cell-wall amino acid at position 3 of the peptide subunit was found to be lysine. The cell-wall peptidoglycan contained alanine, aspartic acid, glutamic acid, glycine, lysine, and serine and thus the peptidoglycan type was concluded to be of A4α type with Lys-Gly-Ser-Asp interpeptide bridge. The genome size was 3.49 Mbp and G + C content of the genome DNA was 69.4 mol%. On the basis of the phenotypic, genomic, and chemotaxonomic characteristics, strain DB0510 T is considered to represent a novel genus and species within the family Dermacoccaceae , for which the name Metallococcus carri gen. nov., sp. nov. is proposed. The type strain of Metallococcus carri is DB0510 T (= KACC 19663  T  = NBRC 113349  T ).
ISSN:0302-8933
1432-072X
DOI:10.1007/s00203-021-02386-4