Dissipation of antibiotic resistance genes in manure-amended agricultural soil

Soil antibiotic resistance due to animal manure application is of great concern in recent years. Little is known about the fate of antibiotic resistance genes (ARGs) in agricultural soils associated with long-term manure application. Here we used soil microcosms to investigate the dissipation of ARG...

Full description

Saved in:
Bibliographic Details
Published in:The Science of the total environment 2021-09, Vol.787, p.147582-147582, Article 147582
Main Authors: He, Liang-Ying, He, Lun-Kai, Gao, Fang-Zhou, Wu, Dai-Ling, Zou, Hai-Yan, Bai, Hong, Zhang, Min, Ying, Guang-Guo
Format: Article
Language:English
Subjects:
Antibiotic resistance genes
Biochar
Dissipation
Manure
Metagenomics
Soil
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Soil antibiotic resistance due to animal manure application is of great concern in recent years. Little is known about the fate of antibiotic resistance genes (ARGs) in agricultural soils associated with long-term manure application. Here we used soil microcosms to investigate the dissipation of ARGs and the change of bacterial community in agricultural soil originated from a vegetable field which had received 24 years' swine manure application. Soil microcosms were conducted at different soil moistures and with or without biochar over a testing period of two years in lab. Results showed that continuous manure application induced an accumulation of ARGs in soil, wherein the dissipation of ARGs differed from those in non-manure amended soil. ARGs persisted in soils at least two years, although their abundance declined gradually. Meanwhile, soil moisture and biochar had significant impact on the fate of ARGs. ARGs dissipated faster in soil with higher moisture. Biochar amendment contributed to the maintenance of bacterial diversity. Within the two years of simulation experiment, biochar enhanced soil ARG retention as they dissipated slowly in the soil amended with biochar. Succession of microbial community may have sustained the transfer and resilience of ARGs. This study provides insight into the dissipation of antibiotic resistance genes in manure-applied agricultural soil. [Display omitted] •ARGs persisted in agricultural soils at least two years after manure application•Dissipation pattern of ARGs in manure applied soil was different to the control.•Abundance of ARGs in manure-applied soils declined gradually within two years.•Moisture and biochar had significant impact on soil ARGs and microbial community.•Biochar amendment maintained the bacterial diversity and enhanced ARG retention.
ISSN:0048-9697
1879-1026
DOI:10.1016/j.scitotenv.2021.147582