Speckled 100 kDa gene in pigs: Alternative splicing, subcellular localization, and response to interferon-α stimulation

Speckled 100 kDa (Sp100) plays an important role in the antiviral immune response, however, little is known about porcine Sp100. In this study, porcine Sp100 was cloned and its response to interferon (IFN) α was identified. We obtained the cDNA (V1) of the gene, SP100, and seven alternative splicing...

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Bibliographic Details
Published in:Gene 2021-07, Vol.791, p.145710-145710, Article 145710
Main Authors: Yang, Xiu-qin, Zhao, Xue-lian, Yu, Hao, Zhang, Jiao, Han, Li-xin, Liu, Di
Format: Article
Language:English
Subjects:
Alternative Splicing - genetics
Animals
Antigens, Nuclear - genetics
Antigens, Nuclear - metabolism
Autoantigens - genetics
Autoantigens - metabolism
Cell Nucleus - metabolism
Cloning, Molecular
DNA, Complementary - metabolism
Gene Expression - genetics
HeLa Cells
Humans
IFN-stimulated response element
Interferon-alpha - genetics
Interferon-alpha - metabolism
Intracellular Space - genetics
Nuclear Proteins - genetics
Polymorphism
Promoter
Promoter Regions, Genetic - genetics
Protein Isoforms - genetics
RNA, Messenger - metabolism
SP100
Swine - genetics
Transcript variant
Transcription Factors - genetics
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Summary:Speckled 100 kDa (Sp100) plays an important role in the antiviral immune response, however, little is known about porcine Sp100. In this study, porcine Sp100 was cloned and its response to interferon (IFN) α was identified. We obtained the cDNA (V1) of the gene, SP100, and seven alternative splicing variants (V2–8). Isoform V1 encoded a 386 amino acid protein and contained a homogeneously-staining region (HSR) domain. Isoforms V3, 4, 6 and 7 were deletion/insertion variants and contained HSR domain as V1. The splicing of porcine SP100 was very complicated and many transcripts existed as revealed by cloning and minigene analyses. Using GFP-fusion constructs isoforms V1, 3, 4, 6 and 7 were localized to nucleus and the nuclear localization signal was identified as PSNRKRR at positions 331–337 of V1. Porcine SP100 was unevenly distributed in all tissues studied and differentially expressed between pigs with different disease-resistance/susceptibilities. Porcine SP100 was strongly increased by IFNα due to the existence of an IFN-stimulated response element in the promoter. A single nucleotide − 70A > C polymorphism enhanced promoter activity. The results provided the basis for determining the role of Sp100 in antiviral responses and may assist in breeding pigs with high disease-resistance. •SP100 and seven alternative splicing variants were characterized in pig.•There existed more alternative splicing variants in SP100 gene.•An interferon-stimulated response element existed in the promoter.•A single nucleotide polymorphism − 70A > C altered promoter activity.
ISSN:0378-1119
1879-0038
DOI:10.1016/j.gene.2021.145710