Withania coagulans extract attenuates oxidative stress-mediated apoptosis of cerebellar purkinje neurons after ischemia/reperfusion injury

Cerebral ischemia/reperfusion (I/R) is known to increase reactive oxygen species (ROS) generation, consequences of oxidative stress (OS), and neuronal death in the susceptible brain areas including the cerebellum. Newly, remarkable attention has been paid to a natural diet with the capability to sca...

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Bibliographic Details
Published in:Metabolic brain disease 2021-10, Vol.36 (7), p.1699-1708
Main Authors: Heidari, Zahra, Mahmoudzadeh-Sagheb, Hamidreza, Sarbishegi, Maryam, Gorgich, Enam Alhagh Charkhat
Format: Article
Language:English
Subjects:
Animals
Antioxidants
Apoptosis
Apoptosis - drug effects
Biochemistry
Biomedical and Life Sciences
Biomedicine
Cerebellum
Distilled water
Injury prevention
Ischemia
Male
Metabolic Diseases
Neurology
Neurons
Neurosciences
Oncology
Original Article
Oxidative stress
Oxidative Stress - drug effects
Plant Extracts - pharmacology
Purkinje cells
Purkinje Cells - drug effects
Purkinje Cells - pathology
Rats
Rats, Wistar
Reactive oxygen species
Reperfusion
Reperfusion Injury - drug therapy
Scavenging
Withania
Withania coagulans
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Summary:Cerebral ischemia/reperfusion (I/R) is known to increase reactive oxygen species (ROS) generation, consequences of oxidative stress (OS), and neuronal death in the susceptible brain areas including the cerebellum. Newly, remarkable attention has been paid to a natural diet with the capability to scavenge ROS. Withania coagulans root extract (WCE) is rich in components with antioxidants properties. Therefore, this study aimed to evaluate the effect of WCE on cerebellar Purkinje cells (PCs) against OS-mediated apoptosis after I/R injury. In this experimental study 64 male adult Wistar rats were randomly divided into 4 groups (n = 16) as follows: control, sham, I/R, and WCE 1000 + I/R. I/R animals were pretreated with daily administration of hydro-alcoholic WCE (1000 mg/kg) or distilled water as a vehicle for 30 days before I/R injury. After 72 h, the animals were sacrificed, the cerebellum tissue was removed and used for biochemical (CAT, SOD, GPx, and MDA levels) and histopathological (Nissl and TUNEL staining) assays. Findings showed that the MDA level and the number of apoptotic neurons significantly increased and viable Purkinje neurons decreased in I/R injury (p 
ISSN:0885-7490
1573-7365
DOI:10.1007/s11011-021-00745-0