Size-Resolved Single Entity Collision Biosensing for Dual Quantification of MicroRNAs in a Single Run

Limited to the accuracy of size resolution, single entity collision biosensing (SECBS) for multiplex immunoassays remains challenging, because it is difficult to get the true value of nanoparticle (NP) sizes based on the current intensity due to the complex movement of NPs on the electrode surface....

Full description

Saved in:
Bibliographic Details
Published in:ACS applied materials & interfaces 2021-05, Vol.13 (19), p.22254-22261
Main Authors: Bai, Yi-Yan, Yang, Yan-Ju, Wu, Zhen, Yang, Xiao-Yan, Lin, Miao, Pang, Dai-Wen, Zhang, Zhi-Ling
Format: Article
Language:English
Subjects:
Biological and Medical Applications of Materials and Interfaces
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Limited to the accuracy of size resolution, single entity collision biosensing (SECBS) for multiplex immunoassays remains challenging, because it is difficult to get the true value of nanoparticle (NP) sizes based on the current intensity due to the complex movement of NPs on the electrode surface. Considering that the size-dependent movement of NPs meanwhile will generate a characteristic current shape, in this work, the huge difference in the current rise time of 5 and 15 nm Pt NPs colliding on an Au ultramicroelectrode (d = 30 μm) was originally used to develop a size-resolved SECBS for multiplex immunoassays of miRNAs. The limit concentration that can be detected was 0.5 fM. Compared with conventional electrochemical biosensors for multiplex immunoassays, for the size-resolved SECBS, one does not need to worry about potential overlapping. Therefore, the proposed method demonstrates a promising potential for the application of SECBS in multiplex immunoassays.
ISSN:1944-8244
1944-8252
DOI:10.1021/acsami.1c04747