Ruthenium(II)-curcumin liposome nanoparticles: Synthesis, characterization, and their effects against cervical cancer

[Display omitted] •Ruthenium(II)-curcumin liposome nanoparticles (RCLNPs) were synthesized using thin layer evaporation method.•Ru-Cur complexes were loaded into RCLNPs for screening effects against HeLa cells.•Nuclear damage studies and Reactive oxygen species (ROS) assay were investigated in HeLa...

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Bibliographic Details
Published in:Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2021-08, Vol.204, p.111773-111773, Article 111773
Main Authors: Lakshmi, Buddolla Anantha, Reddy, Ankireddy Seshadri, Sangubotla, Roopkumar, Hong, Jong Wook, Kim, Sanghyo
Format: Article
Language:English
Subjects:
Curcumin
HeLa cells
Liposome nanoparticles
Metal-based flavonoid
Reactive oxygen species
Ruthenium(II)
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Summary:[Display omitted] •Ruthenium(II)-curcumin liposome nanoparticles (RCLNPs) were synthesized using thin layer evaporation method.•Ru-Cur complexes were loaded into RCLNPs for screening effects against HeLa cells.•Nuclear damage studies and Reactive oxygen species (ROS) assay were investigated in HeLa cells by RCLNPs.•Ru-Cur complexes were thoroughly characterized using 1H, and 13C NMR spectroscopy. Ruthenium complexes have increased the scope for improvement in current cancer treatment by replacing platinum-based drugs. However, to reduce metal-associated toxicity, a biocompatible flavonoid, such as curcumin, is indispensable, as it offers uncompensated therapeutic benefits through formation of complexes. In this study, we synthesized metal-based flavonoid complexes using ruthenium(II) and curcumin by adopting a convenient reflux reaction, represented as Ru-Cur complexes. These complexes were thoroughly characterized using 1H, 13C NMR, XPS, FT-IR, and UV–vis spectroscopy. As curcumin is sparingly soluble in water and has poor chemical stability, we loaded Ru-Cur complexes into liposomes and further formed nanoparticles (NPs) using the thin layer evaporation method. These were named Ru-Cur loaded liposome nanoparticles (RCLNPs). The effects of RCLNPs on cell proliferation was investigated using human cervical cancer cell lines (HeLa). These RCLNPs exhibited significant cytotoxicity in HeLa cells. The anticancer properties of RCLNPs were studied using reactive oxygen species (ROS), LDH, and MTT assays as well as live-dead staining. Nuclear damage studies of RCLNPs were performed in HeLa cells using the Hoechst staining assay.
ISSN:0927-7765
1873-4367
DOI:10.1016/j.colsurfb.2021.111773