Development and Validation of RP-HPLC Method for Vicenin-2, Orientin, Cynaroside, Betulinic Acid, Genistein, and Major Eight Bioactive Constituents with LC-ESI-MS/MS Profiling in Ocimum Genus

Ocimum genus, known as Tulsi or Basil, is a prominent botanical class in Asian culture, especially in India. The leaves have immunomodulatory, antioxidant, stress-relieving, and adaptogenic roles in traditional and modern medicine, with prominent usage in herbal teas and nutraceuticals. An high-perf...

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Bibliographic Details
Published in:Journal of AOAC International 2021-12, Vol.104 (6), p.1634-1651
Main Authors: Girme, Aboli, Bhoj, Prajkta, Saste, Ganesh, Pawar, Sandeep, Mirgal, Amit, Raut, Dipak, Chavan, Machindra, Hingorani, Lal
Format: Article
Language:English
Subjects:
Apigenin
Chromatography, High Pressure Liquid
Flavonoids
Genistein
Glucosides
Luteolin
Ocimum
Ocimum basilicum
Pentacyclic Triterpenes
Plant Extracts
Tandem Mass Spectrometry
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Summary:Ocimum genus, known as Tulsi or Basil, is a prominent botanical class in Asian culture, especially in India. The leaves have immunomodulatory, antioxidant, stress-relieving, and adaptogenic roles in traditional and modern medicine, with prominent usage in herbal teas and nutraceuticals. An high-performance liquid chromatography-photodiode array (HPLC-PDA) method was developed and validated for quantification of vicenin-2, orientin, cynaroside, betulinic acid, genistein with syringic acid, rosmarinic acid, eugenol, carnosic acid, oleanolic acid, ursolic acid, luteolin, and apigenin and was confirmed using a novel electrospray ionisation-mass spectrometry (ESI-MS/MS) method in the Ocimum genus samples. The methodology parameters were developed on an reverse phase (RP) C18 column with a gradient elution of 1 mL/min flow rate for 0.1% o-phosphoric acid and acetonitrile at 210 and 340 nm wavelengths. The validation data for 13 bioactive compounds showed good linearity (r2 > 0.99) with sensitive LOD (0.034-0.684 µg/mL) and LOQ (0.100-2.068 µg/mL) with recoveries (83.66-101.53%). The results of the quantification were found to be precise (RSD,
ISSN:1060-3271
1944-7922
DOI:10.1093/jaoacint/qsab067