Impact of HIV-1 Infection and Antigen Class on T Follicular Helper Cell Responses to Pneumococcal Polysaccharide-Protein Conjugate Vaccine-13

Pneumococcal infections are common and serious complications of HIV-1 disease. Prevention has been compromised by the limited magnitude and quality of Ab responses to T cell-independent type 2 pneumococcal capsular polysaccharides (PPS). The pneumococcal polysaccharide-protein conjugate vaccine-13 (...

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Bibliographic Details
Published in:The Journal of immunology (1950) 2021-05, Vol.206 (10), p.2402-2411
Main Authors: Jha, Vibha, Nicholson, Lindsay K, Gardner, Edward M, Rahkola, Jeremy T, Pratap, Harsh, Scott, James, Borgeson, Mandy, Jacobelli, Jordan, Janoff, Edward N
Format: Article
Language:English
Subjects:
Adaptive Immunity
Adolescent
Adult
AIDS-Related Opportunistic Infections - blood
AIDS-Related Opportunistic Infections - immunology
AIDS-Related Opportunistic Infections - prevention & control
AIDS-Related Opportunistic Infections - virology
Antibodies, Bacterial - blood
Antibodies, Bacterial - immunology
B-Lymphocytes - immunology
Case-Control Studies
Female
HIV-1 - immunology
Humans
Immunogenicity, Vaccine
Inducible T-Cell Co-Stimulator Protein - metabolism
Lymphocyte Activation
Male
Middle Aged
Pneumococcal Infections - blood
Pneumococcal Infections - immunology
Pneumococcal Infections - microbiology
Pneumococcal Infections - prevention & control
Pneumococcal Vaccines - immunology
Streptococcus pneumoniae - immunology
T Follicular Helper Cells - immunology
Treatment Outcome
Vaccination - methods
Vaccines, Conjugate - immunology
Young Adult
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Summary:Pneumococcal infections are common and serious complications of HIV-1 disease. Prevention has been compromised by the limited magnitude and quality of Ab responses to T cell-independent type 2 pneumococcal capsular polysaccharides (PPS). The pneumococcal polysaccharide-protein conjugate vaccine-13 (PCV-13) contains PPS conjugated to the T cell-dependent protein (diphtheria toxoid [DT] [CRM197]). We investigated the differential response to PPS and DT by human Ab-secreting B cells (ASC) after immunization with PCV-13 in newly diagnosed healthy HIV and control adults. The numbers of PPS-specific IgG ASC increased significantly and similarly in HIV and controls. However, DT-specific IgG ASC increased in controls but not HIV subjects. To determine the cellular basis of these disparate responses to DT and PPS, we characterized the frequency and activation of T follicular helper (Tfh) cells, the predominant T cell subset providing B cell help. Expression of inducible T cell costimulator (ICOS), which sustains Tfh function and phenotype, increased significantly among controls, when compared with the HIV group. Increases in ICOS Tfh correlated with changes in T-dependent, DT-specific IgG ASC in controls but not in HIV In contrast, ICOS expression did not correlate with T cell-independent type 2 PPS-specific ASC in either group. Of note, upon optimized ex vivo stimulation, CD4 T cells from HIV subjects differentiated into Tfh cells and formed synapses with Raji B cells at frequencies similar to that of controls. In summary, PCV-13-induced increase in ICOS expression on Tfh was associated with responses to DT, which was compromised in recently diagnosed healthy HIV adults and can be restored ex vivo by providing effective Tfh-differentiating signals.
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.2001133