Exploring the ability of low-level laser irradiation to reduce myonecrosis and increase Myogenin transcription after Bothrops jararacussu envenomation

Envenoming caused by snakebites is a very important neglected tropical disease worldwide. The myotoxic phospholipases present in the bothropic venom disrupt the sarcolemma and compromise the mechanisms of energy production, leading to myonecrosis. Photobiomodulation therapy (PBMT) has been used as a...

Full description

Saved in:
Bibliographic Details
Published in:Photochemical & photobiological sciences 2021-04, Vol.20 (4), p.571-583
Main Authors: Vieira, Willians Fernando, Kenzo-Kagawa, Bruno, Alvares, Lúcia Elvira, Cogo, José Carlos, Baranauskas, Vitor, da Cruz-Höfling, Maria Alice
Format: Article
Language:English
Subjects:
Biochemistry
Biomaterials
Chemistry
Chemistry and Materials Science
Original Papers
Physical Chemistry
Plant Sciences
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Envenoming caused by snakebites is a very important neglected tropical disease worldwide. The myotoxic phospholipases present in the bothropic venom disrupt the sarcolemma and compromise the mechanisms of energy production, leading to myonecrosis. Photobiomodulation therapy (PBMT) has been used as an effective tool to treat diverse cases of injuries, such as snake venom-induced myonecrosis. Based on that, the aim of this study was to analyze the effects of PBMT through low-level laser irradiation (904 nm) on the muscle regeneration after the myonecrosis induced by Bothrops jararacussu snake venom ( Bjssu ) injection, focusing on myogenic regulatory factors expression, such as Pax7 , MyoD , and Myogenin ( MyoG ). Male Swiss mice ( Mus musculus ), 6–8-week-old, weighing 22 ± 3 g were used. Single sub-lethal Bjssu dose or saline was injected into the right mice gastrocnemius muscle. At 3, 24, 48, and 72 h after injections, mice were submitted to PBMT treatment. When finished the periods of 48 and 72 h, mice were euthanized and the right gastrocnemius were collected for analyses. We observed extensive inflammatory infiltrate in all the groups submitted to Bjssu injections. PBMT was able to reduce the myonecrotic area at 48 and 72 h after envenomation. There was a significant increase of MyoG mRNA expression at 72 h after venom injection. The data suggest that beyond the protective effect promoted by PBMT against Bjssu -induced myonecrosis, the low-level laser irradiation was able to stimulate the satellite cells, thus enhancing the muscle repair by improving myogenic differentiation.
ISSN:1474-905X
1474-9092
DOI:10.1007/s43630-021-00041-x