Antimicrobial effects of photodynamic therapy with Fotoenticine on Streptococcus mutans isolated from dental caries

•This study showed photodynamic therapy mediated by Fotoenticine has antimicrobial activity against biofilms formed by clinical strains of S. mutans.•Photodynamic therapy achieved very significant reductions in the number of viable cells. Photodynamic therapy (PDT) is a promising strategy to control...

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Published in:Photodiagnosis and photodynamic therapy 2021-06, Vol.34, p.102303-102303, Article 102303
Main Authors: Terra-Garcia, Maíra, de Souza, Cheyenne Marçal, Ferreira Gonçalves, Nathalia Maria, Pereira, André Henrique Correia, de Barros, Patrícia Pimentel, Borges, Alessandra Bühler, Miyakawa, Walter, Strixino, Juliana Ferreira, Junqueira, Juliana Campos
Format: Article
Language:English
Subjects:
Dental caries
Fotoenticine
Photodynamic therapy
Streptococcus mutans
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Summary:•This study showed photodynamic therapy mediated by Fotoenticine has antimicrobial activity against biofilms formed by clinical strains of S. mutans.•Photodynamic therapy achieved very significant reductions in the number of viable cells. Photodynamic therapy (PDT) is a promising strategy to control cariogenic pathogens, such as Streptococcus mutans. Seeking to reach the total bacterial elimination from dental surfaces, novel photosensitizers have been investigated, such as Fotoenticine (FTC) derived from chlorin e6. The objective of this study was to investigate the photodynamic effects of FTC against several clinical strains of S. mutans. Clinical isolates were obtained from patients with active carious lesions, identified by molecular analysis and subjected to PDT using laser irradiation (660 nm and 39.5 J/cm2) in planktonic and biofilm stages. We identified 11 S. mutans strains from cervical, occlusal and proximal caries. PDT mediated by FTC has totally eliminated the S. mutans cells in planktonic growth for all analyzed strains. In biofilms, PDT with FTC reached statistically significant reductions compared with the non-treated control group, at 5.4, 5.5 and 6.5 Log10 (CFU/mL), respectively, for the strains from proximal, occlusal and cervical caries. The scanning electron microscopy evaluations confirmed that PDT mediated by FTC was able to disaggregate and kill the S. mutans cells adhered to enamel surface, suggesting its potential to disinfect the dental tissues.
ISSN:1572-1000
1873-1597
DOI:10.1016/j.pdpdt.2021.102303