KIF18A knockdown reduces proliferation, migration, invasion and enhances radiosensitivity of esophageal cancer

Kinesin family member 18A (KIF18A) is significantly overexpressed and is related to the poor prognosis of human cancers. However, the function of KIF18A in esophageal cancer (EC) is still unclear. Human EC cell lines were used in this study. KIF18A expression in human tissues was assessed using Gene...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2021-06, Vol.557, p.192-198
Main Authors: Qian, Lu-Xi, Cao, Xiang, Du, Ming-Yu, Ma, Cheng-Xian, Zhu, Hong-Ming, Peng, Yi, Hu, Xin-Yu, He, Xia, Yin, Li
Format: Article
Language:English
Subjects:
Esophageal cancer
IGF2BP3
KIF18A
mRNA stability
Proliferation
Radiosensitivity
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Summary:Kinesin family member 18A (KIF18A) is significantly overexpressed and is related to the poor prognosis of human cancers. However, the function of KIF18A in esophageal cancer (EC) is still unclear. Human EC cell lines were used in this study. KIF18A expression in human tissues was assessed using Gene Expression Profiling Interactive Analysis 2.0 (GEPIA2). The expressions of KIF18A or IGF2BP3 in EC cells were detected using qRT-PCR or WB. Cells were transfected using si-KIF18A, si-IGF2BP3, and plasmid IGF2BP3. The abilities of proliferation, migration, and invasion were detected by EdU, wound-healing, and transwell assay. The interaction between KIF18A and IGF2BP3 was predicted by starBase v3.0 and studied by RIP and RNA stability assay. Colony formation assay was used to reflect the changes of radiosensitivity in EC cells. KIF18A was upregulated in EC, and KIF18A knockdown inhibited EC cell proliferation, migration, invasion, and radioresistance. The prediction in starBase and RIP assay results showed that KIF18A mRNA could bind to IGF2BP3 protein in EC cells. RNA stability assay was performed to confirm that IGF2BP3 affects mRNA stability of KIF18A. Further studies also showed that IGF2BP3 could positively regulate KIF18A on proliferation, migration, invasion, and radioresistance. Our findings first revealed an oncogenic effect of KIF18A in human EC progression. KIF18A expression was associated with radioresistance of EC cells. The binding relationship between KIF18A and IGF2BP3 might influence the mRNA stability of KIF18A in EC cell lines. •Knockdown of KIF18A suppressed cell proliferation and migration in esophageal cancer cells.•Knockdown of KIF18A enhanced the radiosensitivity of esophageal cancer cells.•IGF2BP3 was a direct regulator of KIF18A.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2021.04.020