Effects of Different Continuous Cropping Years on Bacterial Community and Diversity of Cucumber Rhizosphere Soil in Solar-Greenhouse

The rhizosphere soils from 1, 3, 5, and 7 years of cucumber continuous cropping in solar-greenhouse were used as the research objects. The region of bacterial 16S rRNA was analyzed by Illumina MiSeq high-throughput sequencing technology. The effect of continuous cropping years on the microbial commu...

Full description

Saved in:
Bibliographic Details
Published in:Current microbiology 2021-06, Vol.78 (6), p.2380-2390
Main Authors: Li, Yang, Chi, Jingliang, Ao, Jing, Gao, Xiaomei, Liu, Xiaohui, Sun, Yulu, Zhu, Weiwei
Format: Article
Language:English
Subjects:
Abundance
Bacteria
Biomedical and Life Sciences
Biotechnology
Catalase
Chemical properties
Cluster analysis
Clustering
Community composition
Community structure
Continuous cropping
Cucumbers
Ecological succession
Environmental factors
Evolution & development
Greenhouses
Life Sciences
Microbiology
Microorganisms
Next-generation sequencing
Nitrogen
pH effects
Phosphorus
Potassium
Relative abundance
Rhizosphere
rRNA 16S
Soil analysis
Soil chemistry
Soil investigations
Soil microorganisms
Soil properties
Solar power
Sustainable development
Urease
Vegetables
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The rhizosphere soils from 1, 3, 5, and 7 years of cucumber continuous cropping in solar-greenhouse were used as the research objects. The region of bacterial 16S rRNA was analyzed by Illumina MiSeq high-throughput sequencing technology. The effect of continuous cropping years on the microbial community structure and diversity in cucumber soil in the greenhouse was investigated. The physical and chemical properties of soil and the activities of urease and catalase were determined. The results showed that cucumber crop succession for different years affected the community composition of the bacteria at the phylum level, and the abundance of Proteobacteria , Chloroflexi , Gemmatimonadetes , Patescibacteria and Firmicutes gradually increased, while Actinobacteria in the soil significantly decreased. Among the top 15 significantly different genera, with the extension of successive years, the relative abundance of most genera in bacteria decreased after a small increase in year 3. The diversity results indicated that soil samples from continuous cropping for 7 years had the lowest community diversity. PICRUSt analysis showed a decreasing trend in soil bacterial function as the cucumber crop succession age increased. In environmental factor clustering analysis, the soil bacterial community was significantly correlated with pH, available nitrogen (AN), soil urease (SUR) and available phosphorus (AP), and the effect on the bacterial community was expressed as SUR > AP > AN > pH.
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-021-02485-x