N-terminal region of RecQ4 inhibits non-homologous end joining and chromatin association of the Ku heterodimer in Xenopus egg extracts

•RecQ4 may be important in the DNA double-strand break repair pathway choice.•RecQ4 N-terminal inhibited the Xenopus linearized DNA end-joining reaction.•RecQ4 N-terminal region repressed Ku70 DSB-induced chromatin binding. RecQ4, a member of the RecQ helicase family, is required for the maintenance...

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Bibliographic Details
Published in:Gene 2021-06, Vol.787, p.145647-145647, Article 145647
Main Authors: Tsuyama, Takashi, Fujita, Kumiko, Sasaki, Ryosuke, Hamanaka, Shiori, Sotoyama, Yuki, Ogawa, Akira, Kusuzaki, Kana, Azuma, Yutaro, Tada, Shusuke
Format: Article
Language:English
Subjects:
Animals
Chromatin
DNA - metabolism
DNA double-strand breaks
DNA End-Joining Repair
DNA repair
DNA-dependent protein kinase
Ku Autoantigen - antagonists & inhibitors
Ku Autoantigen - metabolism
Protein Binding
RecQ helicase
RecQ Helicases - genetics
RecQ Helicases - metabolism
Rothmund-Thomson syndrome
Xenopus laevis
Xenopus Proteins - physiology
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Summary:•RecQ4 may be important in the DNA double-strand break repair pathway choice.•RecQ4 N-terminal inhibited the Xenopus linearized DNA end-joining reaction.•RecQ4 N-terminal region repressed Ku70 DSB-induced chromatin binding. RecQ4, a member of the RecQ helicase family, is required for the maintenance of genome integrity. RecQ4 has been shown to promote the following two DNA double-strand break (DSB) repair pathways: non-homologous end joining (NHEJ) and homologous recombination (HR). However, its molecular function has not been fully elucidated. In the present study, we aimed to investigate the role of RecQ4 in NHEJ using Xenopus egg extracts. The N-terminal 598 amino acid region of Xenopus RecQ4 (N598), which lacks a central helicase domain and a downstream C-terminal region, was added to the extracts and its effect on the joining of DNA ends was analyzed. We found that N598 inhibited the joining of linearized DNA ends in the extracts. In addition, N598 inhibited DSB-induced chromatin binding of Ku70, which is essential for NHEJ, while the DSB-induced chromatin binding of the HR-associated proteins, replication protein A (RPA) and Rad51, increased upon the addition of N598. These results suggest that RecQ4 possibly influences the choice of the DSB repair pathway by influencing the association of the Ku heterodimer with the DNA ends.
ISSN:0378-1119
1879-0038
DOI:10.1016/j.gene.2021.145647