Beneficial effect of Chlorella pyrenoidosa drink on healthy subjects: A randomized, placebo‐controlled, double‐blind, cross‐over clinical trial

The current study aims to explore the anti‐inflammatory activity of Chlorella pyrenoidosa on RAW 267.4 cells and followed by a cross‐over clinical trial in healthy subjects to check the antioxidant and anti‐aging properties of Chlorella water extract (CWE). For the clinical trial, 44 healthy subject...

Full description

Saved in:
Bibliographic Details
Published in:Journal of food biochemistry 2021-04, Vol.45 (4), p.e13665-n/a
Main Authors: Chiu, Hui‐Fang, Lee, Hsiao‐Ju, Han, Yi‐Chun, Venkatakrishnan, Kamesh, Golovinskaia, Oksana, Wang, Chin‐Kun
Format: Article
Language:English
Subjects:
aging
antioxidant
anti‐inflammatory
Chlorella pyrenoidosa
oxidative stress
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c3655-3e2ef6a1373f0d0df01592711b850b4919149db2e9ce359569439eae2b21f85e3
cites cdi_FETCH-LOGICAL-c3655-3e2ef6a1373f0d0df01592711b850b4919149db2e9ce359569439eae2b21f85e3
container_end_page n/a
container_issue 4
container_start_page e13665
container_title Journal of food biochemistry
container_volume 45
creator Chiu, Hui‐Fang
Lee, Hsiao‐Ju
Han, Yi‐Chun
Venkatakrishnan, Kamesh
Golovinskaia, Oksana
Wang, Chin‐Kun
description The current study aims to explore the anti‐inflammatory activity of Chlorella pyrenoidosa on RAW 267.4 cells and followed by a cross‐over clinical trial in healthy subjects to check the antioxidant and anti‐aging properties of Chlorella water extract (CWE). For the clinical trial, 44 healthy subjects were requested to consume 27 ml of either placebo or CWE for 90 days (phase I) and vice‐versa manner for 90 days (phase II) with 4 weeks of washout period. The RAW 264.7 macrophages treated with Chlorella display potent anti‐inflammatory activity by significantly downregulating (p 
doi_str_mv 10.1111/jfbc.13665
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2504342986</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2504342986</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3655-3e2ef6a1373f0d0df01592711b850b4919149db2e9ce359569439eae2b21f85e3</originalsourceid><addsrcrecordid>eNp9kctuEzEYhS0EomlhwwMgLxFqiq8z4-7aqOWiSmxgPfLlt-LgjFN7pihd9RHY8II8SZ2m7RJv7P_o89GxD0LvKDmhdX1aeWNPKG8a-QLNaCvkXEgqXqIZofXcdZ04QIelrAghTDXiNTrgvJWSsXaG_p7DAD7YoCMG78GOOHm8WMaUIUaNN9sMQwouFY1dDsMvnAa8BB3H5RaXyazqjXKKz3DWg0vrcAvuGG-itmDSv7s_Ng1jTjHuVJcmE6GKJoahzjanUuqYbiBjW7Vga4gx1yhv0CuvY4G3j_sR-nl58WPxZX71_fPXxdnV3PJGyjkHBr7RlLfcE0ecJ1Qq1lJqOkmMUFRRoZxhoCxwqWSjBFeggRlGfSeBH6EPe99NTtcTlLFfh2J3Dx8gTaVnkggumOqain7cow-xM_h-k8Na521PSb-rod_V0D_UUOH3j76TWYN7Rp_-vQJ0D_wOEbb_seq_XZ4v9qb3lsmXUA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2504342986</pqid></control><display><type>article</type><title>Beneficial effect of Chlorella pyrenoidosa drink on healthy subjects: A randomized, placebo‐controlled, double‐blind, cross‐over clinical trial</title><source>EBSCOhost Business Source Ultimate</source><source>Wiley</source><creator>Chiu, Hui‐Fang ; Lee, Hsiao‐Ju ; Han, Yi‐Chun ; Venkatakrishnan, Kamesh ; Golovinskaia, Oksana ; Wang, Chin‐Kun</creator><creatorcontrib>Chiu, Hui‐Fang ; Lee, Hsiao‐Ju ; Han, Yi‐Chun ; Venkatakrishnan, Kamesh ; Golovinskaia, Oksana ; Wang, Chin‐Kun</creatorcontrib><description>The current study aims to explore the anti‐inflammatory activity of Chlorella pyrenoidosa on RAW 267.4 cells and followed by a cross‐over clinical trial in healthy subjects to check the antioxidant and anti‐aging properties of Chlorella water extract (CWE). For the clinical trial, 44 healthy subjects were requested to consume 27 ml of either placebo or CWE for 90 days (phase I) and vice‐versa manner for 90 days (phase II) with 4 weeks of washout period. The RAW 264.7 macrophages treated with Chlorella display potent anti‐inflammatory activity by significantly downregulating (p &lt; .05) the protein expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase‐2 (COX‐2). Whereas, the subjects supplemented with CWE showed improved (p &lt; .05) antioxidant status (TEAC, SOD, CAT, and DHEAs) and lower (p &lt; .05) oxidative stress/aging markers (TBARS and 8‐OHdG) as well as considerably (p &lt; .05) protected liver (by lowering GOT and GPT). Thus, consumption of chlorella could significantly improve the overall health status by suppressing various oxidative stress markers and aging stress markers. Practical applications Chlorella is considered as a popular functional food owing to its rich nutrient value and its array of biological activities. Numerous studies indicated that treatment with Chlorella spp. would considerably lower oxidative stress, inflammation, and regulate immune response which might contribute to anti‐aging property in various cell and animal models. Based on the above information, we expected that Chlorella would be a better contender for the development of a novel anti‐aging agent. Hence, we designed this clinical trial to assess the beneficial effects of Chlorella pyrenoidosa especially anti‐aging. In agreement with our hypothesis, our results also showed that subjected supplemented with Chlorella water extract could significantly improve overall health status by suppressing various oxidative stress markers and aging stress markers. Hence, Chlorella could be developed into a novel anti‐aging agent. In the future, it can be prescribed with standard anti‐aging agents to improve the overall health status of the elderly population. However, large‐scale clinical studies are needed to confirm our statements. Chlorella pyrenoidosa display potent anti‐inflammatory activity on RAW 267.4 cells by downregulating iNOS and COX‐2 expression C. pyrenoidosa treatment improved the antioxidant status and lowered the oxidative stress Consumption of chlorella could significantly improve the overall health status</description><identifier>ISSN: 0145-8884</identifier><identifier>EISSN: 1745-4514</identifier><identifier>DOI: 10.1111/jfbc.13665</identifier><identifier>PMID: 33755227</identifier><language>eng</language><publisher>United States</publisher><subject>aging ; antioxidant ; anti‐inflammatory ; Chlorella pyrenoidosa ; oxidative stress</subject><ispartof>Journal of food biochemistry, 2021-04, Vol.45 (4), p.e13665-n/a</ispartof><rights>2021 Wiley Periodicals LLC.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3655-3e2ef6a1373f0d0df01592711b850b4919149db2e9ce359569439eae2b21f85e3</citedby><cites>FETCH-LOGICAL-c3655-3e2ef6a1373f0d0df01592711b850b4919149db2e9ce359569439eae2b21f85e3</cites><orcidid>0000-0003-1308-2909</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjfbc.13665$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjfbc.13665$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,786,790,27957,27958,50923,51032</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33755227$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chiu, Hui‐Fang</creatorcontrib><creatorcontrib>Lee, Hsiao‐Ju</creatorcontrib><creatorcontrib>Han, Yi‐Chun</creatorcontrib><creatorcontrib>Venkatakrishnan, Kamesh</creatorcontrib><creatorcontrib>Golovinskaia, Oksana</creatorcontrib><creatorcontrib>Wang, Chin‐Kun</creatorcontrib><title>Beneficial effect of Chlorella pyrenoidosa drink on healthy subjects: A randomized, placebo‐controlled, double‐blind, cross‐over clinical trial</title><title>Journal of food biochemistry</title><addtitle>J Food Biochem</addtitle><description>The current study aims to explore the anti‐inflammatory activity of Chlorella pyrenoidosa on RAW 267.4 cells and followed by a cross‐over clinical trial in healthy subjects to check the antioxidant and anti‐aging properties of Chlorella water extract (CWE). For the clinical trial, 44 healthy subjects were requested to consume 27 ml of either placebo or CWE for 90 days (phase I) and vice‐versa manner for 90 days (phase II) with 4 weeks of washout period. The RAW 264.7 macrophages treated with Chlorella display potent anti‐inflammatory activity by significantly downregulating (p &lt; .05) the protein expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase‐2 (COX‐2). Whereas, the subjects supplemented with CWE showed improved (p &lt; .05) antioxidant status (TEAC, SOD, CAT, and DHEAs) and lower (p &lt; .05) oxidative stress/aging markers (TBARS and 8‐OHdG) as well as considerably (p &lt; .05) protected liver (by lowering GOT and GPT). Thus, consumption of chlorella could significantly improve the overall health status by suppressing various oxidative stress markers and aging stress markers. Practical applications Chlorella is considered as a popular functional food owing to its rich nutrient value and its array of biological activities. Numerous studies indicated that treatment with Chlorella spp. would considerably lower oxidative stress, inflammation, and regulate immune response which might contribute to anti‐aging property in various cell and animal models. Based on the above information, we expected that Chlorella would be a better contender for the development of a novel anti‐aging agent. Hence, we designed this clinical trial to assess the beneficial effects of Chlorella pyrenoidosa especially anti‐aging. In agreement with our hypothesis, our results also showed that subjected supplemented with Chlorella water extract could significantly improve overall health status by suppressing various oxidative stress markers and aging stress markers. Hence, Chlorella could be developed into a novel anti‐aging agent. In the future, it can be prescribed with standard anti‐aging agents to improve the overall health status of the elderly population. However, large‐scale clinical studies are needed to confirm our statements. Chlorella pyrenoidosa display potent anti‐inflammatory activity on RAW 267.4 cells by downregulating iNOS and COX‐2 expression C. pyrenoidosa treatment improved the antioxidant status and lowered the oxidative stress Consumption of chlorella could significantly improve the overall health status</description><subject>aging</subject><subject>antioxidant</subject><subject>anti‐inflammatory</subject><subject>Chlorella pyrenoidosa</subject><subject>oxidative stress</subject><issn>0145-8884</issn><issn>1745-4514</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kctuEzEYhS0EomlhwwMgLxFqiq8z4-7aqOWiSmxgPfLlt-LgjFN7pihd9RHY8II8SZ2m7RJv7P_o89GxD0LvKDmhdX1aeWNPKG8a-QLNaCvkXEgqXqIZofXcdZ04QIelrAghTDXiNTrgvJWSsXaG_p7DAD7YoCMG78GOOHm8WMaUIUaNN9sMQwouFY1dDsMvnAa8BB3H5RaXyazqjXKKz3DWg0vrcAvuGG-itmDSv7s_Ng1jTjHuVJcmE6GKJoahzjanUuqYbiBjW7Vga4gx1yhv0CuvY4G3j_sR-nl58WPxZX71_fPXxdnV3PJGyjkHBr7RlLfcE0ecJ1Qq1lJqOkmMUFRRoZxhoCxwqWSjBFeggRlGfSeBH6EPe99NTtcTlLFfh2J3Dx8gTaVnkggumOqain7cow-xM_h-k8Na521PSb-rod_V0D_UUOH3j76TWYN7Rp_-vQJ0D_wOEbb_seq_XZ4v9qb3lsmXUA</recordid><startdate>202104</startdate><enddate>202104</enddate><creator>Chiu, Hui‐Fang</creator><creator>Lee, Hsiao‐Ju</creator><creator>Han, Yi‐Chun</creator><creator>Venkatakrishnan, Kamesh</creator><creator>Golovinskaia, Oksana</creator><creator>Wang, Chin‐Kun</creator><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-1308-2909</orcidid></search><sort><creationdate>202104</creationdate><title>Beneficial effect of Chlorella pyrenoidosa drink on healthy subjects: A randomized, placebo‐controlled, double‐blind, cross‐over clinical trial</title><author>Chiu, Hui‐Fang ; Lee, Hsiao‐Ju ; Han, Yi‐Chun ; Venkatakrishnan, Kamesh ; Golovinskaia, Oksana ; Wang, Chin‐Kun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3655-3e2ef6a1373f0d0df01592711b850b4919149db2e9ce359569439eae2b21f85e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>aging</topic><topic>antioxidant</topic><topic>anti‐inflammatory</topic><topic>Chlorella pyrenoidosa</topic><topic>oxidative stress</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chiu, Hui‐Fang</creatorcontrib><creatorcontrib>Lee, Hsiao‐Ju</creatorcontrib><creatorcontrib>Han, Yi‐Chun</creatorcontrib><creatorcontrib>Venkatakrishnan, Kamesh</creatorcontrib><creatorcontrib>Golovinskaia, Oksana</creatorcontrib><creatorcontrib>Wang, Chin‐Kun</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of food biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chiu, Hui‐Fang</au><au>Lee, Hsiao‐Ju</au><au>Han, Yi‐Chun</au><au>Venkatakrishnan, Kamesh</au><au>Golovinskaia, Oksana</au><au>Wang, Chin‐Kun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Beneficial effect of Chlorella pyrenoidosa drink on healthy subjects: A randomized, placebo‐controlled, double‐blind, cross‐over clinical trial</atitle><jtitle>Journal of food biochemistry</jtitle><addtitle>J Food Biochem</addtitle><date>2021-04</date><risdate>2021</risdate><volume>45</volume><issue>4</issue><spage>e13665</spage><epage>n/a</epage><pages>e13665-n/a</pages><issn>0145-8884</issn><eissn>1745-4514</eissn><notes>ObjectType-Article-2</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-News-1</notes><notes>ObjectType-Feature-3</notes><notes>content type line 23</notes><abstract>The current study aims to explore the anti‐inflammatory activity of Chlorella pyrenoidosa on RAW 267.4 cells and followed by a cross‐over clinical trial in healthy subjects to check the antioxidant and anti‐aging properties of Chlorella water extract (CWE). For the clinical trial, 44 healthy subjects were requested to consume 27 ml of either placebo or CWE for 90 days (phase I) and vice‐versa manner for 90 days (phase II) with 4 weeks of washout period. The RAW 264.7 macrophages treated with Chlorella display potent anti‐inflammatory activity by significantly downregulating (p &lt; .05) the protein expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase‐2 (COX‐2). Whereas, the subjects supplemented with CWE showed improved (p &lt; .05) antioxidant status (TEAC, SOD, CAT, and DHEAs) and lower (p &lt; .05) oxidative stress/aging markers (TBARS and 8‐OHdG) as well as considerably (p &lt; .05) protected liver (by lowering GOT and GPT). Thus, consumption of chlorella could significantly improve the overall health status by suppressing various oxidative stress markers and aging stress markers. Practical applications Chlorella is considered as a popular functional food owing to its rich nutrient value and its array of biological activities. Numerous studies indicated that treatment with Chlorella spp. would considerably lower oxidative stress, inflammation, and regulate immune response which might contribute to anti‐aging property in various cell and animal models. Based on the above information, we expected that Chlorella would be a better contender for the development of a novel anti‐aging agent. Hence, we designed this clinical trial to assess the beneficial effects of Chlorella pyrenoidosa especially anti‐aging. In agreement with our hypothesis, our results also showed that subjected supplemented with Chlorella water extract could significantly improve overall health status by suppressing various oxidative stress markers and aging stress markers. Hence, Chlorella could be developed into a novel anti‐aging agent. In the future, it can be prescribed with standard anti‐aging agents to improve the overall health status of the elderly population. However, large‐scale clinical studies are needed to confirm our statements. Chlorella pyrenoidosa display potent anti‐inflammatory activity on RAW 267.4 cells by downregulating iNOS and COX‐2 expression C. pyrenoidosa treatment improved the antioxidant status and lowered the oxidative stress Consumption of chlorella could significantly improve the overall health status</abstract><cop>United States</cop><pmid>33755227</pmid><doi>10.1111/jfbc.13665</doi><tpages>0</tpages><orcidid>https://orcid.org/0000-0003-1308-2909</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0145-8884
ispartof Journal of food biochemistry, 2021-04, Vol.45 (4), p.e13665-n/a
issn 0145-8884
1745-4514
language eng
recordid cdi_proquest_miscellaneous_2504342986
source EBSCOhost Business Source Ultimate; Wiley
subjects aging
antioxidant
anti‐inflammatory
Chlorella pyrenoidosa
oxidative stress
title Beneficial effect of Chlorella pyrenoidosa drink on healthy subjects: A randomized, placebo‐controlled, double‐blind, cross‐over clinical trial
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-09-21T14%3A10%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Beneficial%20effect%20of%20Chlorella%20pyrenoidosa%20drink%20on%20healthy%20subjects:%20A%20randomized,%20placebo%E2%80%90controlled,%20double%E2%80%90blind,%20cross%E2%80%90over%20clinical%20trial&rft.jtitle=Journal%20of%20food%20biochemistry&rft.au=Chiu,%20Hui%E2%80%90Fang&rft.date=2021-04&rft.volume=45&rft.issue=4&rft.spage=e13665&rft.epage=n/a&rft.pages=e13665-n/a&rft.issn=0145-8884&rft.eissn=1745-4514&rft_id=info:doi/10.1111/jfbc.13665&rft_dat=%3Cproquest_cross%3E2504342986%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c3655-3e2ef6a1373f0d0df01592711b850b4919149db2e9ce359569439eae2b21f85e3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2504342986&rft_id=info:pmid/33755227&rfr_iscdi=true