Eosinophils increase macrophage ability to control intracellular Leishmania amazonensis infection via PGD2 paracrine activity in vitro

•Eosinophils enhance macrophage ability to control intracellular L. amazonensis infection in vitro.•Eosinophil-driven mechanism depends on a paracrine activity mediated by soluble factor(s).•Eosinophil-secreted PGD2 mediates enhanced macrophage ability to manage L. amazonensis infection.•Macrophage...

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Bibliographic Details
Published in:Cellular immunology 2021-05, Vol.363, p.104316-104316, Article 104316
Main Authors: da Silva Marques, Patrícia, da Fonseca-Martins, Alessandra M., Carneiro, Monique Pacheco Duarte, Amorim, Natália R.T., de Pão, Camila R. Rodrigues, Canetti, Claudio, Diaz, Bruno L., de Matos Guedes, Herbert L., Bandeira-Melo, Christianne
Format: Article
Language:English
Subjects:
Eosinophil
L. amazonensis
Macrophage
Prostaglandin D2
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Summary:•Eosinophils enhance macrophage ability to control intracellular L. amazonensis infection in vitro.•Eosinophil-driven mechanism depends on a paracrine activity mediated by soluble factor(s).•Eosinophil-secreted PGD2 mediates enhanced macrophage ability to manage L. amazonensis infection.•Macrophage DP2 receptors mediate the effect of eosinophil-derived PGD2.•Eosinophil/PGD2 may be adjuvant therapeutic option in L. amazonensis-driven diseases. Clinical and experimental studies have described eosinophil infiltration in Leishmania amazonensis infection sites, positioning eosinophils strategically adjacent to the protozoan-infected macrophages in cutaneous leishmaniasis. Here, by co-culturing mouse eosinophils with L. amazonensis-infected macrophages, we studied the impact of eosinophils on macrophage ability to regulate intracellular L. amazonensis infection. Eosinophils prevented the increase in amastigote numbers within macrophages by a mechanism dependent on a paracrine activity mediated by eosinophil-derived prostaglandin (PG) D2 acting on DP2 receptors. Exogenous PGD2 mimicked eosinophil-mediated effect on managing L. amazonensis intracellular infection by macrophages and therefore may function as a complementary tool for therapeutic intervention in L. amazonensis-driven cutaneous leishmaniasis.
ISSN:0008-8749
1090-2163
DOI:10.1016/j.cellimm.2021.104316