Protective effect of fermented Diospyros lotus L. extracts against the high glucose‐induced apoptosis of MIN6 cells

Date plum persimmon (Diospyros lotus L.) is a fruit crop from the Ebenaceae family. Its microorganism‐fermented extract (DPEML) was shown to exhibit a hypoglycemic effect in our previous work. Here, we investigated the effects of DPEML fermented by Microbacterium flavum YM18‐098 and Lactobacillus pl...

Full description

Saved in:
Bibliographic Details
Published in:Journal of food biochemistry 2021-04, Vol.45 (4), p.e13685-n/a
Main Authors: Yin, Hao, Yan, Huan‐huan, Qin, Chen‐qiang, Li, Hai‐rong, Li, Xue, Ren, Di‐feng
Format: Article
Language:English
Subjects:
apoptosis
cell viability
date plum persimmon extracts co‐fermented by Microbacterium flavum YM18‐098 and Lactobacillus plantarum B7
high glucose‐induced MIN6 cell
type 2 diabetes mellitus
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Date plum persimmon (Diospyros lotus L.) is a fruit crop from the Ebenaceae family. Its microorganism‐fermented extract (DPEML) was shown to exhibit a hypoglycemic effect in our previous work. Here, we investigated the effects of DPEML fermented by Microbacterium flavum YM18‐098 and Lactobacillus plantarum B7 on the high glucose‐induced apoptosis of MIN6 cells and explored its potential cell protective mechanisms. DPEML ameliorated the apoptosis of MIN6 cells cultured under high glucose conditions, thereby improving cell viability. DPEML upregulated the Bcl‐2/Bax mRNA ratio to obstruct an intrinsic apoptotic pathway and concomitantly downregulated the expression of the apoptosis‐linked proteins, AIF, and Cyt‐C, in high glucose‐induced MIN6 cells. Furthermore, DPEML promoted the insulin secretion of MIN6 cells grown under chronically high‐glucose conditions by upregulating Ins mRNA expression. In summary, our study suggested that DPEML is a promising functional food for the development of therapeutics for the treatment of Type 2 diabetes mellitus. Practical applications We investigated the effects of DPEML fermented by Microbacterium flavum YM18‐098 and Lactobacillus plantarum B7 on the high glucose‐induced apoptosis of MIN6 cells and explored its potential cell protective mechanisms. DPEML ameliorated the apoptosis of MIN6 cells cultured under high glucose conditions, thereby improving cell viability. DPEML upregulated the Bcl‐2/Bax mRNA ratio to obstruct an intrinsic apoptotic pathway and concomitantly downregulated the expression of the apoptosis‐linked proteins, AIF and Cyt‐C, in high glucose‐induced MIN6 cells. Furthermore, DPEML promoted the insulin secretion of MIN6 cells grown under chronically high‐glucose conditions by upregulating Ins mRNA expression. We suggested that DPEML is a promising functional food for the development of therapeutics for the treatment of Type 2 diabetes mellitus.
ISSN:0145-8884
1745-4514
DOI:10.1111/jfbc.13685