Production of antimicrobial metabolites against pathogenic bacteria and yeasts by Fusarium oxysporum in submerged culture processes

The antimicrobial activity of the metabolites produced by Fusarium oxysporum PR-33 in submerged culture was evaluated against Gram-positive and Gram-negative bacteria and yeasts. Metabolites were determined by HPLC–DAD-MS/MS. An extract was obtained following the removal of mycelium by centrifugatio...

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Bibliographic Details
Published in:Bioprocess and biosystems engineering 2021-06, Vol.44 (6), p.1321-1332
Main Authors: Poleto, Liliane, da Rosa, Letícia Osório, Fontana, Roselei Claudete, Rodrigues, Eliseu, Poletto, Édina, Baldo, Guilherme, Paesi, Suelen, Sales-Campos, Ceci, Camassola, Marli
Format: Article
Language:English
Subjects:
Acids
Antiinfectives and antibacterials
Antimicrobial activity
Antimicrobial agents
Bacteria
Bioreactors
Biotechnology
Cell culture
Centrifugation
Chemistry
Chemistry and Materials Science
Cytotoxicity
Environmental Engineering/Biotechnology
Food Science
Fusaric acid
Fusarium oxysporum
Gram-negative bacteria
High-performance liquid chromatography
Industrial and Production Engineering
Industrial Chemistry/Chemical Engineering
Liquid chromatography
Metabolites
Research Paper
Toxicity
Yeast
Yeasts
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Summary:The antimicrobial activity of the metabolites produced by Fusarium oxysporum PR-33 in submerged culture was evaluated against Gram-positive and Gram-negative bacteria and yeasts. Metabolites were determined by HPLC–DAD-MS/MS. An extract was obtained following the removal of mycelium by centrifugation and lyophilisation of the supernatant. The compounds in this extract demonstrated broad-spectrum antimicrobial action, with rates of inhibition between 60 and 80%, depending on the species and extract tested. The major compounds of the extracts were identified as fusarinolic acid and its isomer [56.9% flask extract (FE)] and 59.2% bioreactor extract (BE), dehydrofusaric acid (35.7% FE and 31.6% BE), and fusaric acid (6.5% FE and 1.1% BE). Fusaric acid has been shown to be responsible for antimicrobial activity. The cytotoxicity of the extracts was evaluated in culture of HEK-293 and SH-SY5Y animal cells and toxicity of these extracts was verified even in the lowest tested concentrations. Therefore, our results indicate that the compounds identified exhibit potential as antimicrobial agents. Graphic abstract
ISSN:1615-7591
1615-7605
DOI:10.1007/s00449-021-02538-2