Loading…
An integrative approach uncovers transcriptome‐wide determinants of mRNA stability regulation in Saccharomyces cerevisiae
mRNA degradation rate is one of the key stages of gene expression regulation in eukaryotic cells. To date, intertwined processes of post‐transcriptional control have been widely investigated, but focused rather on the examination of mechanisms controlling stability of particular protein‐coding trans...
Saved in:
Published in: | The FEBS journal 2021-06, Vol.288 (11), p.3418-3423 |
---|---|
Main Authors: | , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Summary: | mRNA degradation rate is one of the key stages of gene expression regulation in eukaryotic cells. To date, intertwined processes of post‐transcriptional control have been widely investigated, but focused rather on the examination of mechanisms controlling stability of particular protein‐coding transcripts. Currently, a wealth of information from structural, biochemical, and high‐throughput studies makes it tempting to define general rules governing mRNA stability that could be considered as versatile and valid on a genome‐wide scale. Basu et al. analyzed multiple experimental and computational data on Saccharomyces cerevisiae mRNA half‐lives as well as on secondary structures and protein‐binding sites within transcripts, and collated it with available structures of ribonucleases, that is, enzymes responsible for mRNA degradation. This approach allowed to conclude how particular mRNA features such as lengths of unstructured terminal or internal regions or sequestration into ribonucleoprotein complexes impact half‐lives of protein‐coding transcripts and to define genome‐scale principles of mRNA stability control in yeast.
Post‐transcriptional events appreciably contribute to the regulation of gene expression in Saccharomyces cerevisiae. mRNA stability depends on cis elements (transcript structure) and factors acting in trans, for example, exo‐/endoribonucleases and RNA‐binding proteins/ribonucleoproteins exerting mRNA degradation and fulfilling protective functions, respectively. A new study by Basu et al. provides insights into mRNA half‐life regulation determinants in yeast on a global scale, based on a comprehensive, multi‐angle analysis of computational and experimental data.
Comment on: https://doi.org/10.1111/febs.15670. |
---|---|
ISSN: | 1742-464X 1742-4658 |
DOI: | 10.1111/febs.15742 |