BnaA02.NIP6;1a encodes a boron transporter required for plant development under boron deficiency in Brassica napus

Boron (B) is an essential micronutrient for the plant normal growth. In Arabidopsis, NIP6;1 is a boric acid channel required for the proper distribution of boric acid, especially in the nodal regions of shoots. BnaA02.NIP6;1a, a homologous gene of AtNIP6;1 in Brassica napus, was reported to play a k...

Full description

Saved in:
Bibliographic Details
Published in:Plant physiology and biochemistry 2021-04, Vol.161, p.36-45
Main Authors: Song, Ge, Li, Xueping, Munir, Raheel, Khan, Ali Raza, Azhar, Wardah, Khan, Sulaiman, Gan, Yinbo
Format: Article
Language:English
Subjects:
Arabidopsis
BnaA02.NIP6
Boron - metabolism
Boron transport
Brassica napus
Brassica napus - genetics
Brassica napus - metabolism
Floral organ
Gene Expression Regulation, Plant
Membrane Transport Proteins - metabolism
Plant Development
Plant Proteins - genetics
Plant Proteins - metabolism
Seedling
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Boron (B) is an essential micronutrient for the plant normal growth. In Arabidopsis, NIP6;1 is a boric acid channel required for the proper distribution of boric acid, especially in the nodal regions of shoots. BnaA02.NIP6;1a, a homologous gene of AtNIP6;1 in Brassica napus, was reported to play a key role in B transport activity. However, little is known about the other functions of BnaA02.NIP6;1a in Brassica napus. In this study, we found that BnaA02.NIP6; 1a was localized in both plasma membrane and cytoplasm, which was different from that in Arabidopsis. The transgenic Arabidopsis plant containing a BnaA02.NIP6;1a promoter driven GUS reporter gene displayed strong GUS activity in roots, stems, leaves, especially in buds and open flowers, which are different from the expression pattern from its homologous gene in Arabidopsis. Silencing BnaA02.NIP6;1a repressed vegetative growth under B-deficient condition in Brassica napus. More importantly, knockdown of BnaA02.NIP6;1a in rapeseed resulted in the reduction of boron accumulation in the flower under boron deficiency and lead to severe sterility, which has not yet been reported before. Furthermore, nip6;1 mutant in Arabidopsis only showed the loss of apical dominance phenotype under boron deficiency at reproductive stage, whereas BnaA02.NIP6;1 RNAi lines exhibited large amounts of abnormal development of the inflorescence as compared with the wild type under boron limitation. Taken together, our results demonstrate that BnaA02.NIP6;1a encodes a boron transporter required for plant development under boron deficiency in Brassica napus, which shows its novel and diverse function in rapeseed compared with model plant Arabidopsis. •Distinct BnaA02.NIP6;1a GUS activity was observed in Arabidopsis compared with that of Arabidopsis NIP6;1.•Overexpressing BnaA02.NIP6;1a rescued the seedling phenotype of nip6;1-3 in Arabidopsis under B limitation.•Knockdown of BnaA02.NIP6;1a in Brassica napus increased the sensitivity of the RNAi line seedlings to B deficiency.•Novel role of B transporter BnaA02.NIP6; 1a for the inflorescence development in Brassica napus.•Knockdown of BnaA02.NIP6;1a inhibits the cell enlargement of floral organs in Brassica napus.
ISSN:0981-9428
1873-2690
DOI:10.1016/j.plaphy.2021.01.041