Ligustrazine induces viability, suppresses apoptosis and autophagy of retinal ganglion cells with ischemia/reperfusion injury through the PI3K/Akt/mTOR signaling pathway

Ligustrazine, an alkaloid monomer extracted from Chuanxiong Rhizoma, has the function of protecting nerve cells. However, the effect and mechanism of ligustrazine on retinal ischemia/reperfusion (I/R) injury still need to be clarified. In our study, retinal ganglion cells (RGC-5) were used to establ...

Full description

Saved in:
Bibliographic Details
Published in:Bioengineered 2021-01, Vol.12 (1), p.507-515
Main Authors: Du, Hong-yan, Wang, Rong, Li, Jian-liang, Luo, Huang, Xie, Xiao-yan, Yan, Ran, Jian, Yue-ling, Cai, Jin-ying
Format: Article
Language:English
Subjects:
Animals
Apoptosis - drug effects
autophagy
Autophagy - drug effects
Cell Survival - drug effects
ischemia/reperfusion injury
Ligustrazine
Phosphatidylinositol 3-Kinase - metabolism
PI3K/Akt/mTOR signaling pathway
Proto-Oncogene Proteins c-akt - metabolism
Pyrazines - pharmacology
Rats
Reperfusion Injury - metabolism
Research Paper
retinal ganglion cells
Retinal Ganglion Cells - cytology
Retinal Ganglion Cells - drug effects
Signal Transduction - drug effects
TOR Serine-Threonine Kinases - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Ligustrazine, an alkaloid monomer extracted from Chuanxiong Rhizoma, has the function of protecting nerve cells. However, the effect and mechanism of ligustrazine on retinal ischemia/reperfusion (I/R) injury still need to be clarified. In our study, retinal ganglion cells (RGC-5) were used to establish a retinal I/R injury model by anaerobic cultivation. Cell viability, autophagy, and apoptosis were evaluated by cell counting kit 8 assay, transmission electron microscopy, and TUNEL staining after treatment with ligustrazine, PI3K inhibitor Ly294002, and/or mTOR inhibitor rapamycin, respectively. Besides, the levels of PI3K/Akt/mTOR pathway and autophagy-related proteins were determined by western blot. Moreover, one-way ANOVA was adopted for inter-group comparisons of measurement data. Our results demonstrated that low-concentration ligustrazine significantly enhanced cell viability and suppressed cell autophagy and apoptosis of RGC-5 cells after I/R injury, suggesting the protective effect of low-concentration ligustrazine on retinal I/R injury. Moreover, the alleviating effect of ligustrazine on RGC-5 with retinal I/R injury was mechanistically associated with the activation of the PI3K/Akt/mTOR pathway. In conclusion, low-concentration ligustrazine has a significant protective effect on RGC-5 cells with retinal I/R injury by activating the PI3K/Akt/mTOR pathway.
ISSN:2165-5979
2165-5987
DOI:10.1080/21655979.2021.1880060