Identification and characterization of SlbHLH, SlDof and SlWRKY transcription factors interacting with SlDPD gene involved in costunolide biosynthesis in Saussurea lappa

The genes involved in costunolide biosynthesis in Saussurea lappa have been identified recently by our lab. However, the study of transcriptional regulators of these genes was lacking for better opportunities for engineering the pharmacologically important biosynthetic pathway. Therefore, we cloned...

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Bibliographic Details
Published in:International journal of biological macromolecules 2021-03, Vol.173, p.146-159
Main Authors: Thakur, Vasundhara, Bains, Savita, Kaur, Ravneet, Singh, Kashmir
Format: Article
Language:English
Subjects:
Amino Acid Sequence
bHLH
Binding Sites
Biosynthetic Pathways - genetics
Carboxy-Lyases - genetics
Carboxy-Lyases - metabolism
Costunolide
Diphosphomevalonate decarboxylase
Dof
Gene Expression Profiling
Gene Expression Regulation, Plant
Gene Regulatory Networks
Phylogeny
Plant Proteins - genetics
Plant Proteins - metabolism
Promoter Regions, Genetic
Protein Binding
Saussurea - enzymology
Saussurea - genetics
Sequence Alignment
Sequence Homology, Amino Acid
Sesquiterpene lactone
Sesquiterpenes - metabolism
Transcription Factors - genetics
Transcription Factors - metabolism
Transcriptome
Two-Hybrid System Techniques
WRKY
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Summary:The genes involved in costunolide biosynthesis in Saussurea lappa have been identified recently by our lab. However, the study of transcriptional regulators of these genes was lacking for better opportunities for engineering the pharmacologically important biosynthetic pathway. Therefore, we cloned the promoter region of diphosphomevalonate decarboxylase gene (DPD) and analyzed its cis-acting regulatory elements to reveal the potential transcription factor (TF) binding sites for Dof, bHLH and WRKY family proteins in the gene promoter. The transcriptome study approach followed by the hidden Markov model based search, digital gene expression, co-expression network analysis, conserved domain properties and evolutionary analyses were carried out to screen out seven putative TFs for the DPD-TF interaction studies. Yeast one-hybrid assays were performed and three TFs were reported, namely, SlDOF2, SlbHLH3 and SlWRKY2 from Dof, bHLH and WRKY families, respectively that interacted positively with the DPD gene of the costunolide biosynthetic pathway. The tissue specific relative gene expression studies also supported the linked co-expression of the gene and its interacting TFs The present report will improve the understanding of transcriptional regulation pattern of costunolide biosynthetic pathway.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2021.01.114