Influence of microcurrent on the modulation of remodelling genes in a wound healing assay

The literature has shown the beneficial effects of microcurrent (MC) therapy on tissue repair. We investigated if the application of MC at 10 μA/90 s could modulate the expression of remodeling genes transforming growth factor beta (Tgfb) , connective tissue growth factor ( Ctgf ), insulin-like grow...

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Bibliographic Details
Published in:Molecular biology reports 2021-02, Vol.48 (2), p.1233-1241
Main Authors: Silva, Daniela Fernanda Dezotti, de Oliveira Fujii, Lucas, Chiarotto, Gabriela Bortolança, de Oliveira, Camila Andrea, de Andrade, Thiago Antônio Moretti, de Oliveira, Alexandre Leite Rodrigues, Esquisatto, Marcelo Augusto Marretto, Mendonça, Fernanda Aparecida Sampaio, dos Santos, Gláucia Maria Tech, de Aro, Andrea Aparecida
Format: Article
Language:English
Subjects:
Animal Anatomy
Animal Biochemistry
Animals
Biomedical and Life Sciences
Cell adhesion & migration
Cell migration
Cell Movement - radiation effects
Cell viability
Connective tissue growth factor
Connective Tissue Growth Factor - genetics
Connective tissues
Electric Stimulation Therapy
Fibroblasts
Fibromodulin - genetics
Fibronectin
Fibronectins - genetics
Gene expression
Gene Expression Regulation - radiation effects
Growth factors
Histology
Humans
Insulin
Insulin-like growth factor I
Insulin-Like Growth Factor I - genetics
Life Sciences
Mice
Morphology
NIH 3T3 Cells
Original Article
Tenascin
Tenascin - genetics
Tenascin C
Tissue engineering
Transforming Growth Factor beta1 - genetics
Transforming growth factor-b
Wound healing
Wound Healing - genetics
Wound Healing - radiation effects
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Summary:The literature has shown the beneficial effects of microcurrent (MC) therapy on tissue repair. We investigated if the application of MC at 10 μA/90 s could modulate the expression of remodeling genes transforming growth factor beta (Tgfb) , connective tissue growth factor ( Ctgf ), insulin-like growth factor 1 ( Igf1 ), tenascin C ( Tnc ), Fibronectin ( Fn1 ), Scleraxis ( Scx ), Fibromodulin ( Fmod ) and tenomodulin in NIH/3T3 fibroblasts in a wound healing assay. The cell migration was analyzed between days 0 and 4 in both fibroblasts (F) and fibroblasts + MC (F+MC) groups. On the 4th day, cell viability and gene expression were also analyzed after daily MC application. Higher expression of Ctgf and lower expression of Tnc and Fmod , respectively, were observed in the F+MC group in relation to F group (p 
ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-021-06135-0