Formulation development of cream with mupirocin and essential oils for eradication of biofilm mediated antimicrobial resistance

Staphylococcus aureus ( S.aureus ) is both a colonizer as well as a human pathogen that causes a variety of diseases. Mupirocin is a topical antimicrobial agent which is very effective against S.aureus infection. However, treating the S.aureus infection using mupirocin could be complicated due to bi...

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Bibliographic Details
Published in:Archives of microbiology 2021-05, Vol.203 (4), p.1707-1715
Main Authors: Sundaramoorthy, Mahalakshmi, Karuppaiah, Arjunan, Nithyanth, Munusamy, Baberoselin, Rajandurai, Ramesh, Santhanam, Geetha, Natesan, Veintramuthu, Sankar
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
Antimicrobial agents
Antimicrobial resistance
Biochemistry
Biofilms
Biofilms - drug effects
Biomedical and Life Sciences
Biotechnology
Carbon monoxide
Cell Biology
Chronic infection
Cinnamon
Drug Resistance, Bacterial - drug effects
Ecology
Essential oils
Eucalyptus
Eucalyptus Oil - pharmacology
Eugenol
Eugenol - pharmacology
Humans
Infections
Life Sciences
Microbial Ecology
Microbial Sensitivity Tests
Microbiology
Mupirocin
Mupirocin - pharmacology
Oils & fats
Oils, Volatile - pharmacology
Original Paper
Skin Cream - chemistry
Skin Cream - pharmacology
Staphylococcal Infections - drug therapy
Staphylococcus aureus - drug effects
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Summary:Staphylococcus aureus ( S.aureus ) is both a colonizer as well as a human pathogen that causes a variety of diseases. Mupirocin is a topical antimicrobial agent which is very effective against S.aureus infection. However, treating the S.aureus infection using mupirocin could be complicated due to biofilm formation. Consequently, resistance to mupirocin occurs and leads to chronic infection. The combination of mupirocin with a compound that has biofilm eradicating effect would be an ideal solution for effectively treating biofilm infections. Therefore, in this study, we have investigated the biofilm inhibitory and eradication effect of mupirocin with three essential oils (Cinnamon Oil (CO), Eugenol (EU) and Eucalyptus Oil (EO)) against sessile S.aureus . From these preliminary results, it was found that the mupirocin-CO (0.2 µg/ml-5.218 mg/ml) combination has a better synergistic antibiofilm effect against sessile S.aureus and the fractional inhibitory concentration index was found to be 0.458. The best combination of mupirocin with CO was loaded into a non-greasy O/W cream. The physico-chemical and microbiological evaluations were carried out for the prepared cream. The prepared cream has better biofilm eradication activity (40%) when compared to a marketed cream (20%).
ISSN:0302-8933
1432-072X
DOI:10.1007/s00203-020-02175-5